Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18777 | 3' | -58 | NC_004683.1 | + | 21831 | 0.67 | 0.533671 |
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Target: 5'- -cGGUCGuugaaccaccGGUcUGCCCGCgGG-CUGGg -3' miRNA: 3'- aaCCAGU----------CCAaGCGGGUGgCCaGACC- -5' |
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| 18777 | 3' | -58 | NC_004683.1 | + | 42591 | 0.67 | 0.492521 |
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Target: 5'- -cGGUCGGGgccggUCGUCCGgCUGGggUGGu -3' miRNA: 3'- aaCCAGUCCa----AGCGGGU-GGCCagACC- -5' |
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| 18777 | 3' | -58 | NC_004683.1 | + | 40194 | 0.67 | 0.482459 |
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Target: 5'- aUGGUCGcGGccgCGUCCgGCUGGUCUGu -3' miRNA: 3'- aACCAGU-CCaa-GCGGG-UGGCCAGACc -5' |
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| 18777 | 3' | -58 | NC_004683.1 | + | 21788 | 0.68 | 0.472497 |
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Target: 5'- gUUGGUCGGGUaCGgCCACgGGcggcgUUGGu -3' miRNA: 3'- -AACCAGUCCAaGCgGGUGgCCa----GACC- -5' |
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| 18777 | 3' | -58 | NC_004683.1 | + | 20130 | 0.7 | 0.370613 |
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Target: 5'- --cGUCGGGcccaaccgCGCCguauCACCGGUCUGGc -3' miRNA: 3'- aacCAGUCCaa------GCGG----GUGGCCAGACC- -5' |
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| 18777 | 3' | -58 | NC_004683.1 | + | 39946 | 0.76 | 0.152633 |
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Target: 5'- gUGGgugCAGGUuggCGCUCGCCGG-CUGGu -3' miRNA: 3'- aACCa--GUCCAa--GCGGGUGGCCaGACC- -5' |
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| 18777 | 3' | -58 | NC_004683.1 | + | 16717 | 1.07 | 0.000813 |
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Target: 5'- cUUGGUCAGGUUCGCCCACCGGUCUGGc -3' miRNA: 3'- -AACCAGUCCAAGCGGGUGGCCAGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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