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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18781 | 3' | -52.8 | NC_004683.1 | + | 21880 | 0.66 | 0.830872 |
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Target: 5'- gGGCAGacguacaCCGGUGGcgcUGAgcggGCUGCUGGUGc -3' miRNA: 3'- -UUGUCa------GGCCACU---ACU----UGGCGACUAC- -5' |
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| 18781 | 3' | -52.8 | NC_004683.1 | + | 21751 | 0.66 | 0.80222 |
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Target: 5'- gGGCAGaCCGGUGGUucaacGACCGCggccaccGAUGg -3' miRNA: 3'- -UUGUCaGGCCACUAc----UUGGCGa------CUAC- -5' |
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| 18781 | 3' | -52.8 | NC_004683.1 | + | 8021 | 0.67 | 0.761446 |
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Target: 5'- uGCAG-CCGGUGAUGcgcACCGCggucGAg- -3' miRNA: 3'- uUGUCaGGCCACUACu--UGGCGa---CUac -5' |
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| 18781 | 3' | -52.8 | NC_004683.1 | + | 18350 | 0.68 | 0.729356 |
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Target: 5'- uGGCAG-CCGGUGA--GGCCGCgacggucuaccUGAUGg -3' miRNA: 3'- -UUGUCaGGCCACUacUUGGCG-----------ACUAC- -5' |
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| 18781 | 3' | -52.8 | NC_004683.1 | + | 2468 | 0.7 | 0.58378 |
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Target: 5'- gAACGGUCCGGUGuucgcguGCaUGCUGGUGc -3' miRNA: 3'- -UUGUCAGGCCACuacu---UG-GCGACUAC- -5' |
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| 18781 | 3' | -52.8 | NC_004683.1 | + | 17617 | 1.06 | 0.002797 |
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Target: 5'- gAACAGUCCGGUGAUGAACCGCUGAUGc -3' miRNA: 3'- -UUGUCAGGCCACUACUUGGCGACUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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