Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18785 | 3' | -55.3 | NC_004683.1 | + | 294 | 0.66 | 0.751414 |
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Target: 5'- cGCUCGGagugGGAUCCgugaccucCGGggcCCCAGUcaUCg -3' miRNA: 3'- -CGAGCCa---UCUAGGa-------GCC---GGGUCA--AGa -5' |
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| 18785 | 3' | -55.3 | NC_004683.1 | + | 29596 | 0.66 | 0.74104 |
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Target: 5'- gGCaUGGaauacgAGAUCgUCGGCCCAGa--- -3' miRNA: 3'- -CGaGCCa-----UCUAGgAGCCGGGUCaaga -5' |
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| 18785 | 3' | -55.3 | NC_004683.1 | + | 17756 | 0.66 | 0.730557 |
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Target: 5'- cGUUCGGggucGGAcUCCuUCGGCaCCAGcgUCUc -3' miRNA: 3'- -CGAGCCa---UCU-AGG-AGCCG-GGUCa-AGA- -5' |
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| 18785 | 3' | -55.3 | NC_004683.1 | + | 44372 | 0.66 | 0.719976 |
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Target: 5'- uGCcCGGgcGAUCgUCGGCgCGGaUCg -3' miRNA: 3'- -CGaGCCauCUAGgAGCCGgGUCaAGa -5' |
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| 18785 | 3' | -55.3 | NC_004683.1 | + | 4840 | 0.67 | 0.687761 |
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Target: 5'- uGCUCcauccgggcGUGGAccUCgUCGGCCCGGUUg- -3' miRNA: 3'- -CGAGc--------CAUCU--AGgAGCCGGGUCAAga -5' |
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| 18785 | 3' | -55.3 | NC_004683.1 | + | 11046 | 0.67 | 0.644114 |
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Target: 5'- aGUUCGGU--GUCCagucggggcucaUCGGCCCAGacgUCa -3' miRNA: 3'- -CGAGCCAucUAGG------------AGCCGGGUCa--AGa -5' |
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| 18785 | 3' | -55.3 | NC_004683.1 | + | 41821 | 0.67 | 0.644114 |
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Target: 5'- cGCg-GGUAGGUCaUCGGCUCGGgcaggUCUc -3' miRNA: 3'- -CGagCCAUCUAGgAGCCGGGUCa----AGA- -5' |
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| 18785 | 3' | -55.3 | NC_004683.1 | + | 41960 | 0.69 | 0.514432 |
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Target: 5'- cGCUCGGgcGccCCUCGGCCUgccAGUg-- -3' miRNA: 3'- -CGAGCCauCuaGGAGCCGGG---UCAaga -5' |
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| 18785 | 3' | -55.3 | NC_004683.1 | + | 18776 | 1.09 | 0.000994 |
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Target: 5'- cGCUCGGUAGAUCCUCGGCCCAGUUCUu -3' miRNA: 3'- -CGAGCCAUCUAGGAGCCGGGUCAAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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