Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18787 | 3' | -54.1 | NC_004683.1 | + | 37268 | 0.75 | 0.320514 |
Target: 5'- gGGGCCAGUgagUCGUCaCaacggccgcuaucuaGCGCGGGCGg -3' miRNA: 3'- gUCCGGUCAa--AGCAGcG---------------UGCGCUUGC- -5' |
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18787 | 3' | -54.1 | NC_004683.1 | + | 53515 | 0.75 | 0.294604 |
Target: 5'- -cGGCCAGguugUCGUC-CGCGuCGAACGg -3' miRNA: 3'- guCCGGUCaa--AGCAGcGUGC-GCUUGC- -5' |
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18787 | 3' | -54.1 | NC_004683.1 | + | 33256 | 0.76 | 0.266245 |
Target: 5'- -cGGCUAGccagUCGUCGCAcgcuuCGCGGACGg -3' miRNA: 3'- guCCGGUCaa--AGCAGCGU-----GCGCUUGC- -5' |
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18787 | 3' | -54.1 | NC_004683.1 | + | 20957 | 1.08 | 0.001735 |
Target: 5'- cCAGGCCAGUUUCGUCGCACGCGAACGc -3' miRNA: 3'- -GUCCGGUCAAAGCAGCGUGCGCUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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