Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18815 | 5' | -53.5 | NC_004683.1 | + | 19298 | 0.66 | 0.796851 |
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Target: 5'- ---cCGGUGGUguGGAUGGcCUC-CGa -3' miRNA: 3'- aauuGCUACCAguCCUGCCaGAGcGC- -5' |
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| 18815 | 5' | -53.5 | NC_004683.1 | + | 39942 | 0.67 | 0.776917 |
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Target: 5'- --uGCGGUGGgugCAGGuuGGcgCUCGCc -3' miRNA: 3'- aauUGCUACCa--GUCCugCCa-GAGCGc -5' |
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| 18815 | 5' | -53.5 | NC_004683.1 | + | 6298 | 0.67 | 0.776917 |
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Target: 5'- -gGugGGcUGGUUAGGacGCGGUUUCGaCGu -3' miRNA: 3'- aaUugCU-ACCAGUCC--UGCCAGAGC-GC- -5' |
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| 18815 | 5' | -53.5 | NC_004683.1 | + | 50918 | 0.67 | 0.735303 |
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Target: 5'- --cGCGAcGGUCucgcGACGG-CUCGCGa -3' miRNA: 3'- aauUGCUaCCAGuc--CUGCCaGAGCGC- -5' |
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| 18815 | 5' | -53.5 | NC_004683.1 | + | 45571 | 0.68 | 0.724604 |
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Target: 5'- -cGACGAgccGGUCggugcGGGGCGcGUCgcgCGCGa -3' miRNA: 3'- aaUUGCUa--CCAG-----UCCUGC-CAGa--GCGC- -5' |
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| 18815 | 5' | -53.5 | NC_004683.1 | + | 3949 | 0.68 | 0.680987 |
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Target: 5'- gUGGCGGccgUGGUCGaacugcaccuuGGGCGGcgaCUCGCGg -3' miRNA: 3'- aAUUGCU---ACCAGU-----------CCUGCCa--GAGCGC- -5' |
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| 18815 | 5' | -53.5 | NC_004683.1 | + | 41821 | 0.71 | 0.536332 |
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Target: 5'- --cGCGGguaGGUCAucggcucGGGCaGGUCUCGCGg -3' miRNA: 3'- aauUGCUa--CCAGU-------CCUG-CCAGAGCGC- -5' |
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| 18815 | 5' | -53.5 | NC_004683.1 | + | 38869 | 1.06 | 0.002683 |
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Target: 5'- cUUAACGAUGGUCAGGACGGUCUCGCGc -3' miRNA: 3'- -AAUUGCUACCAGUCCUGCCAGAGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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