miRNA: 3'- aGCCGGa--------GCgGC-AGCCGCuCGcGCAG- -5'
miRNA: 3'- -AGCCGGAGCgGCAGCCGcuCG---CGCag -5'
miRNA: 3'- agCCGGa-GCGGCag-----CCGcuCGCGCAG- -5'
miRNA: 3'- aGCCGga-GCGGCaGCCGCUcGCGC-AG- -5'
Results 81 - 84 of 84 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18818 | 3' | -63.3 | NC_004683.1 | + | 28641 | 0.66 | 0.361185 |
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Target: 5'- aCGGCCgagacuuggCGgCGcUCGGCGuGC-CGUCu -3' miRNA: 3'- aGCCGGa--------GCgGC-AGCCGCuCGcGCAG- -5' |
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| 18818 | 3' | -63.3 | NC_004683.1 | + | 23047 | 0.66 | 0.361185 |
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Target: 5'- gUCGGUCUCGgUGUCGGCcuGCucgGCGg- -3' miRNA: 3'- -AGCCGGAGCgGCAGCCGcuCG---CGCag -5' |
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| 18818 | 3' | -63.3 | NC_004683.1 | + | 40237 | 0.66 | 0.369312 |
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Target: 5'- -aGGCCcgCGCCGagcugcaGGC--GCGCGUCg -3' miRNA: 3'- agCCGGa-GCGGCag-----CCGcuCGCGCAG- -5' |
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| 18818 | 3' | -63.3 | NC_004683.1 | + | 18200 | 0.66 | 0.369312 |
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Target: 5'- gCGGUaggUGCCGgCGGCGAcCGCGaUCg -3' miRNA: 3'- aGCCGga-GCGGCaGCCGCUcGCGC-AG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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