Results 81 - 84 of 84 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18818 | 3' | -63.3 | NC_004683.1 | + | 53600 | 0.66 | 0.33756 |
Target: 5'- cCGGCCgcgucuUCGCCGcCGaGCugcGGCGCGaUCa -3' miRNA: 3'- aGCCGG------AGCGGCaGC-CGc--UCGCGC-AG- -5' |
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18818 | 3' | -63.3 | NC_004683.1 | + | 53713 | 0.66 | 0.322446 |
Target: 5'- gCGGCCagGUCG-CGGCGGGCcugaucGCGcCg -3' miRNA: 3'- aGCCGGagCGGCaGCCGCUCG------CGCaG- -5' |
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18818 | 3' | -63.3 | NC_004683.1 | + | 55974 | 0.68 | 0.273562 |
Target: 5'- gUUGGCCUUGaauaCGgUGGCGAcGCGCGg- -3' miRNA: 3'- -AGCCGGAGCg---GCaGCCGCU-CGCGCag -5' |
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18818 | 3' | -63.3 | NC_004683.1 | + | 56442 | 0.66 | 0.329939 |
Target: 5'- aUGGCCUuggugcUGUCGUgGGUGAGCagccCGUCg -3' miRNA: 3'- aGCCGGA------GCGGCAgCCGCUCGc---GCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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