miRNA: 3'- uACUGa--CGUGG--UGGACG------UCUAGUUGG- -5'
miRNA: 3'- uaCUGaCGUGGUGGa------CGUCUA-GUUGG- -5'
miRNA: 3'- uacUGACGUGgUG-GACGUCUAGuUGG- -5'
miRNA: 3'- -UAC--UGACGUGGUgGaCGUCUAGU---UGG- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18827 | 5' | -52.7 | NC_004683.1 | + | 52655 | 0.7 | 0.605542 |
|
Target: 5'- uUGACcacGCGCCggAUCUGCucggucAGAUCGACCa -3' miRNA: 3'- uACUGa--CGUGG--UGGACG------UCUAGUUGG- -5' |
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| 18827 | 5' | -52.7 | NC_004683.1 | + | 56601 | 0.7 | 0.582953 |
|
Target: 5'- -aGGCaGCACCACCgcgggccGCGGGUaCGACUa -3' miRNA: 3'- uaCUGaCGUGGUGGa------CGUCUA-GUUGG- -5' |
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| 18827 | 5' | -52.7 | NC_004683.1 | + | 56622 | 0.68 | 0.684811 |
|
Target: 5'- --aGCUGCACaGCgCUGCAGGUCcAUCg -3' miRNA: 3'- uacUGACGUGgUG-GACGUCUAGuUGG- -5' |
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| 18827 | 5' | -52.7 | NC_004683.1 | + | 56975 | 0.66 | 0.792381 |
|
Target: 5'- gGUGccGCUGCAUCGgC-GCAGGUCGgggGCCu -3' miRNA: 3'- -UAC--UGACGUGGUgGaCGUCUAGU---UGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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