Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18827 | 5' | -52.7 | NC_004683.1 | + | 41031 | 0.66 | 0.821748 |
Target: 5'- aGUGACcucgGCGCCuACCUcgccggcgaGCuGAUCAGCa -3' miRNA: 3'- -UACUGa---CGUGG-UGGA---------CGuCUAGUUGg -5' |
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18827 | 5' | -52.7 | NC_004683.1 | + | 47545 | 0.66 | 0.840285 |
Target: 5'- cUGGCUGCACgACCgu--GGUC-GCCg -3' miRNA: 3'- uACUGACGUGgUGGacguCUAGuUGG- -5' |
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18827 | 5' | -52.7 | NC_004683.1 | + | 43716 | 0.66 | 0.840285 |
Target: 5'- -cGACcGCAUCGC--GCGGAUCGugCa -3' miRNA: 3'- uaCUGaCGUGGUGgaCGUCUAGUugG- -5' |
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18827 | 5' | -52.7 | NC_004683.1 | + | 45877 | 0.75 | 0.316195 |
Target: 5'- cAUGugUGCGCCgAUCUgucGCAGAUUGACCc -3' miRNA: 3'- -UACugACGUGG-UGGA---CGUCUAGUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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