Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18835 | 3' | -56.3 | NC_004683.1 | + | 53940 | 0.66 | 0.66163 |
Target: 5'- -aUCGGGcccuucucaucgcCGGGGUGCACGaGGUGGGg -3' miRNA: 3'- gaGGCCCa------------GCCCUACGUGCaCCAUUU- -5' |
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18835 | 3' | -56.3 | NC_004683.1 | + | 18489 | 0.66 | 0.622183 |
Target: 5'- -aCCGGGgcaGGGAUGUgGCGUaGGUGGc -3' miRNA: 3'- gaGGCCCag-CCCUACG-UGCA-CCAUUu -5' |
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18835 | 3' | -56.3 | NC_004683.1 | + | 46228 | 0.71 | 0.375179 |
Target: 5'- gUCCGGGggcgccgcagucaCGGGAccgcaUGUACGUGGUGu- -3' miRNA: 3'- gAGGCCCa------------GCCCU-----ACGUGCACCAUuu -5' |
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18835 | 3' | -56.3 | NC_004683.1 | + | 24077 | 0.71 | 0.361341 |
Target: 5'- -gCCGGGgCGGGcgGUgGCGUGGUGAu -3' miRNA: 3'- gaGGCCCaGCCCuaCG-UGCACCAUUu -5' |
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18835 | 3' | -56.3 | NC_004683.1 | + | 33292 | 0.74 | 0.242925 |
Target: 5'- -gCUGGGU-GGGAUGCACGUaGGUGc- -3' miRNA: 3'- gaGGCCCAgCCCUACGUGCA-CCAUuu -5' |
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18835 | 3' | -56.3 | NC_004683.1 | + | 47463 | 1.06 | 0.001261 |
Target: 5'- aCUCCGGGUCGGGAUGCACGUGGUAAAc -3' miRNA: 3'- -GAGGCCCAGCCCUACGUGCACCAUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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