Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18837 | 3' | -55.5 | NC_004683.1 | + | 35906 | 0.66 | 0.741545 |
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Target: 5'- gCGUUCCAUCCGcacgacgcgccugaGCCUgGcGGGAc--- -3' miRNA: 3'- gGCAAGGUAGGC--------------UGGAgC-CCCUcuuu -5' |
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| 18837 | 3' | -55.5 | NC_004683.1 | + | 7346 | 0.66 | 0.702543 |
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Target: 5'- aCGUU-CGUCUGGCCgUCGGGGGu--- -3' miRNA: 3'- gGCAAgGUAGGCUGG-AGCCCCUcuuu -5' |
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| 18837 | 3' | -55.5 | NC_004683.1 | + | 14892 | 0.67 | 0.648471 |
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Target: 5'- gCUGaUCCcgCCGAUCgUgGGGGAGAc- -3' miRNA: 3'- -GGCaAGGuaGGCUGG-AgCCCCUCUuu -5' |
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| 18837 | 3' | -55.5 | NC_004683.1 | + | 9397 | 0.7 | 0.508913 |
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Target: 5'- ----cCCAUUCGGCC-CGGGGAGGc- -3' miRNA: 3'- ggcaaGGUAGGCUGGaGCCCCUCUuu -5' |
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| 18837 | 3' | -55.5 | NC_004683.1 | + | 42984 | 0.7 | 0.475267 |
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Target: 5'- gCGUaCCGUCCGccuucagcccgugcACCUCGGGGucGAGGa -3' miRNA: 3'- gGCAaGGUAGGC--------------UGGAGCCCCu-CUUU- -5' |
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| 18837 | 3' | -55.5 | NC_004683.1 | + | 49598 | 1.09 | 0.001178 |
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Target: 5'- aCCGUUCCAUCCGACCUCGGGGAGAAAg -3' miRNA: 3'- -GGCAAGGUAGGCUGGAGCCCCUCUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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