Results 1 - 11 of 11 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18841 | 5' | -53 | NC_004683.1 | + | 752 | 0.66 | 0.840574 |
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Target: 5'- uGCcgCGCCG-CGAUGAcCACgaacUCGAa -3' miRNA: 3'- gCGa-GCGGCaGCUACUaGUGa---AGCUa -5' |
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| 18841 | 5' | -53 | NC_004683.1 | + | 21499 | 0.67 | 0.79361 |
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Target: 5'- gCGC-CGCUGUCGGUGAcCAUccCGAUc -3' miRNA: 3'- -GCGaGCGGCAGCUACUaGUGaaGCUA- -5' |
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| 18841 | 5' | -53 | NC_004683.1 | + | 30819 | 0.67 | 0.79361 |
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Target: 5'- aGCg-GCCGUCGAUGG--GCUacUCGAUg -3' miRNA: 3'- gCGagCGGCAGCUACUagUGA--AGCUA- -5' |
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| 18841 | 5' | -53 | NC_004683.1 | + | 44447 | 0.67 | 0.773539 |
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Target: 5'- -uCUCGCCGUCGAUGuaGUCgGCcgUCGGc -3' miRNA: 3'- gcGAGCGGCAGCUAC--UAG-UGa-AGCUa -5' |
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| 18841 | 5' | -53 | NC_004683.1 | + | 8164 | 0.68 | 0.752869 |
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Target: 5'- uCGUcggCGCCGUCGAgcgGGUCACcUCa-- -3' miRNA: 3'- -GCGa--GCGGCAGCUa--CUAGUGaAGcua -5' |
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| 18841 | 5' | -53 | NC_004683.1 | + | 56473 | 0.68 | 0.731694 |
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Target: 5'- gCGCUguUGCCaugCGGUGAUCGCacugUCGAUg -3' miRNA: 3'- -GCGA--GCGGca-GCUACUAGUGa---AGCUA- -5' |
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| 18841 | 5' | -53 | NC_004683.1 | + | 40874 | 0.69 | 0.654975 |
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Target: 5'- gGcCUCGCCGUCGgcGAgCGCgUCGGUc -3' miRNA: 3'- gC-GAGCGGCAGCuaCUaGUGaAGCUA- -5' |
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| 18841 | 5' | -53 | NC_004683.1 | + | 20076 | 0.7 | 0.621544 |
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Target: 5'- gGUUCGCacaGUCGAUGGUgGCcUCGGc -3' miRNA: 3'- gCGAGCGg--CAGCUACUAgUGaAGCUa -5' |
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| 18841 | 5' | -53 | NC_004683.1 | + | 23000 | 0.7 | 0.610406 |
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Target: 5'- gGCU-GCCGUCGuUGAUCACcagCGAc -3' miRNA: 3'- gCGAgCGGCAGCuACUAGUGaa-GCUa -5' |
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| 18841 | 5' | -53 | NC_004683.1 | + | 39960 | 0.73 | 0.420914 |
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Target: 5'- gCGCUCGCCGgcuGGUGAcUCGCgUCGAg -3' miRNA: 3'- -GCGAGCGGCag-CUACU-AGUGaAGCUa -5' |
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| 18841 | 5' | -53 | NC_004683.1 | + | 54604 | 1.06 | 0.003157 |
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Target: 5'- gCGCUCGCCGUCGAUGAUCACUUCGAUc -3' miRNA: 3'- -GCGAGCGGCAGCUACUAGUGAAGCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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