Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18846 | 5' | -52.9 | NC_004683.1 | + | 2915 | 0.66 | 0.8202 |
Target: 5'- cGGCgUugGCgUCGGAgcggaGCUGGGCGAGUUc -3' miRNA: 3'- -CUG-AugCG-GGUCUa----CGACUUGCUCAA- -5' |
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18846 | 5' | -52.9 | NC_004683.1 | + | 39658 | 0.66 | 0.810567 |
Target: 5'- cGCUACGUCgAGAUGCUugGAACGucGGa- -3' miRNA: 3'- cUGAUGCGGgUCUACGA--CUUGC--UCaa -5' |
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18846 | 5' | -52.9 | NC_004683.1 | + | 4148 | 0.72 | 0.470943 |
Target: 5'- uGAaguCGCUCAGAucgcugaUGCUGAACGAGUa -3' miRNA: 3'- -CUgauGCGGGUCU-------ACGACUUGCUCAa -5' |
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18846 | 5' | -52.9 | NC_004683.1 | + | 2315 | 1.05 | 0.002694 |
Target: 5'- cGACUACGCCCAGAUGCUGAACGAGUUc -3' miRNA: 3'- -CUGAUGCGGGUCUACGACUUGCUCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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