Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1888 | 3' | -52.1 | NC_001347.2 | + | 70434 | 0.68 | 0.978862 |
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Target: 5'- gAGCAGuUGACUGAGAUUGagaucccGgGAGCGu -3' miRNA: 3'- gUUGUCuACUGGCUCUGAU-------CgCUCGC- -5' |
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| 1888 | 3' | -52.1 | NC_001347.2 | + | 114300 | 0.69 | 0.971504 |
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Target: 5'- gCGACAGA-GGCUGAGAUUua-GAGCGc -3' miRNA: 3'- -GUUGUCUaCUGGCUCUGAucgCUCGC- -5' |
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| 1888 | 3' | -52.1 | NC_001347.2 | + | 94480 | 0.69 | 0.962119 |
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Target: 5'- gCGGCGGGUgGACCGGGA--AGCcGGCGg -3' miRNA: 3'- -GUUGUCUA-CUGGCUCUgaUCGcUCGC- -5' |
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| 1888 | 3' | -52.1 | NC_001347.2 | + | 1975 | 0.7 | 0.960012 |
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Target: 5'- gGACAGGaaaaagcgugugaucUGACCGAGGCgaagcauGUGGGUGg -3' miRNA: 3'- gUUGUCU---------------ACUGGCUCUGau-----CGCUCGC- -5' |
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| 1888 | 3' | -52.1 | NC_001347.2 | + | 152422 | 0.7 | 0.954784 |
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Target: 5'- -uGCAGGUGGCCGAGcGCcugGGCGccguGCGc -3' miRNA: 3'- guUGUCUACUGGCUC-UGa--UCGCu---CGC- -5' |
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| 1888 | 3' | -52.1 | NC_001347.2 | + | 751 | 0.73 | 0.861814 |
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Target: 5'- gCGGCGGGUGgguguguGCCGGGugUGucGCGGGCGu -3' miRNA: 3'- -GUUGUCUAC-------UGGCUCugAU--CGCUCGC- -5' |
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| 1888 | 3' | -52.1 | NC_001347.2 | + | 30996 | 0.75 | 0.786771 |
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Target: 5'- aGACGGGgucGA-CGAGGCUGGUGAGCGc -3' miRNA: 3'- gUUGUCUa--CUgGCUCUGAUCGCUCGC- -5' |
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| 1888 | 3' | -52.1 | NC_001347.2 | + | 221011 | 0.75 | 0.764408 |
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Target: 5'- aCAGCAGcgGAUUGAGACaacagugacaaaaGGCGAGCGa -3' miRNA: 3'- -GUUGUCuaCUGGCUCUGa------------UCGCUCGC- -5' |
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| 1888 | 3' | -52.1 | NC_001347.2 | + | 208858 | 0.76 | 0.69981 |
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Target: 5'- -cACcGGUGGCCGAG-CUAuGCGAGCGg -3' miRNA: 3'- guUGuCUACUGGCUCuGAU-CGCUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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