Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1888 | 5' | -61.9 | NC_001347.2 | + | 59933 | 0.66 | 0.76429 |
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Target: 5'- uUUGCgGCGUaCAGGAgccggCgCGGGAGCu -3' miRNA: 3'- gAGCGaCGCG-GUCCUa----GgGCCCUCGu -5' |
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| 1888 | 5' | -61.9 | NC_001347.2 | + | 162893 | 0.66 | 0.755322 |
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Target: 5'- uCUCGCccgcgcuguggGCGCUgcuGGGUCgaGGGAGCGa -3' miRNA: 3'- -GAGCGa----------CGCGGu--CCUAGggCCCUCGU- -5' |
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| 1888 | 5' | -61.9 | NC_001347.2 | + | 94482 | 0.66 | 0.746258 |
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Target: 5'- -cCGCgGCGgguggaCCGGGAagccggcggaggUCgCCGGGAGCAg -3' miRNA: 3'- gaGCGaCGC------GGUCCU------------AG-GGCCCUCGU- -5' |
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| 1888 | 5' | -61.9 | NC_001347.2 | + | 145878 | 0.67 | 0.699763 |
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Target: 5'- gUCGCUuaGCCaccaccuggacgGGGGggaCCGGGGGCGg -3' miRNA: 3'- gAGCGAcgCGG------------UCCUag-GGCCCUCGU- -5' |
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| 1888 | 5' | -61.9 | NC_001347.2 | + | 38036 | 0.67 | 0.690283 |
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Target: 5'- cCUCGCcggcccgagcGCGCCGGGGagaagaaccucuUCCCGGGccccGCGu -3' miRNA: 3'- -GAGCGa---------CGCGGUCCU------------AGGGCCCu---CGU- -5' |
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| 1888 | 5' | -61.9 | NC_001347.2 | + | 151684 | 0.68 | 0.613503 |
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Target: 5'- cCUCGCgGCGCgAGGAcuccaugagCCUGGGcGCGc -3' miRNA: 3'- -GAGCGaCGCGgUCCUa--------GGGCCCuCGU- -5' |
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| 1888 | 5' | -61.9 | NC_001347.2 | + | 68043 | 0.68 | 0.603892 |
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Target: 5'- --aGCUGCucCCGGGAUCCUGGcGcAGCGa -3' miRNA: 3'- gagCGACGc-GGUCCUAGGGCC-C-UCGU- -5' |
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| 1888 | 5' | -61.9 | NC_001347.2 | + | 115916 | 0.73 | 0.351737 |
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Target: 5'- -cCGCgaacUGCGCCAGGccuUCCCGGG-GCu -3' miRNA: 3'- gaGCG----ACGCGGUCCu--AGGGCCCuCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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