Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18895 | 5' | -54.9 | NC_004683.1 | + | 47758 | 0.66 | 0.742454 |
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Target: 5'- gGCGGGACCGccgauGAAGGUGUGC--UUGa -3' miRNA: 3'- gCGUCCUGGUc----CUUCCACGUGucAAC- -5' |
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| 18895 | 5' | -54.9 | NC_004683.1 | + | 48349 | 0.66 | 0.710369 |
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Target: 5'- aGCGGcGACCAGcGAAcGGUGCcguucgcguuguGCGGUg- -3' miRNA: 3'- gCGUC-CUGGUC-CUU-CCACG------------UGUCAac -5' |
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| 18895 | 5' | -54.9 | NC_004683.1 | + | 48598 | 0.66 | 0.696228 |
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Target: 5'- gCGCGGGGCCgaucaccgucAGGAgacggcgacguaggAGGUGCcCAGcUGa -3' miRNA: 3'- -GCGUCCUGG----------UCCU--------------UCCACGuGUCaAC- -5' |
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| 18895 | 5' | -54.9 | NC_004683.1 | + | 7613 | 0.67 | 0.654373 |
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Target: 5'- gGCAGGugCAGGGGcaccggccgucguGGUGCggGCAGc-- -3' miRNA: 3'- gCGUCCugGUCCUU-------------CCACG--UGUCaac -5' |
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| 18895 | 5' | -54.9 | NC_004683.1 | + | 30572 | 0.67 | 0.644394 |
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Target: 5'- aGCGGGAUCAGGgcGGUGacCGCGu--- -3' miRNA: 3'- gCGUCCUGGUCCuuCCAC--GUGUcaac -5' |
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| 18895 | 5' | -54.9 | NC_004683.1 | + | 21730 | 0.69 | 0.556109 |
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Target: 5'- uGCAGaACCucguGGAGGGUGCGCGa--- -3' miRNA: 3'- gCGUCcUGGu---CCUUCCACGUGUcaac -5' |
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| 18895 | 5' | -54.9 | NC_004683.1 | + | 691 | 0.69 | 0.534485 |
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Target: 5'- gCGCAGuGAuCCAGGAGGcGUGUACGuGUc- -3' miRNA: 3'- -GCGUC-CU-GGUCCUUC-CACGUGU-CAac -5' |
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| 18895 | 5' | -54.9 | NC_004683.1 | + | 52345 | 0.7 | 0.48187 |
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Target: 5'- aCGCAGG-CCGGugcGAGGGUGCACcGg-- -3' miRNA: 3'- -GCGUCCuGGUC---CUUCCACGUGuCaac -5' |
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| 18895 | 5' | -54.9 | NC_004683.1 | + | 23985 | 0.7 | 0.471643 |
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Target: 5'- aGCAGGGCCAcac-GGUGCGCAacGUUGc -3' miRNA: 3'- gCGUCCUGGUccuuCCACGUGU--CAAC- -5' |
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| 18895 | 5' | -54.9 | NC_004683.1 | + | 22493 | 1.08 | 0.001312 |
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Target: 5'- uCGCAGGACCAGGAAGGUGCACAGUUGc -3' miRNA: 3'- -GCGUCCUGGUCCUUCCACGUGUCAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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