Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18909 | 3' | -47.7 | NC_004683.1 | + | 31170 | 1.1 | 0.004685 |
Target: 5'- cUGACAUCUGGCAAACAGUUUUCCCUGa -3' miRNA: 3'- -ACUGUAGACCGUUUGUCAAAAGGGAC- -5' |
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18909 | 3' | -47.7 | NC_004683.1 | + | 2146 | 0.73 | 0.757521 |
Target: 5'- gUGGCcgugCUGGCGGGCcuggGGUggUCCCUGa -3' miRNA: 3'- -ACUGua--GACCGUUUG----UCAaaAGGGAC- -5' |
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18909 | 3' | -47.7 | NC_004683.1 | + | 37600 | 0.68 | 0.952469 |
Target: 5'- gUGACGUC-GGCGGGCAccacGUaggUUUCCCa- -3' miRNA: 3'- -ACUGUAGaCCGUUUGU----CA---AAAGGGac -5' |
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18909 | 3' | -47.7 | NC_004683.1 | + | 14016 | 0.67 | 0.9686 |
Target: 5'- cGACGg--GGCGAACAGUUUcucggUCCUc- -3' miRNA: 3'- aCUGUagaCCGUUUGUCAAA-----AGGGac -5' |
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18909 | 3' | -47.7 | NC_004683.1 | + | 24232 | 0.66 | 0.980394 |
Target: 5'- aUGACGUCacgcacUGGC-AGCGGUgggCCCg- -3' miRNA: 3'- -ACUGUAG------ACCGuUUGUCAaaaGGGac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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