Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18911 | 3' | -55.2 | NC_004683.1 | + | 44697 | 0.66 | 0.73381 |
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Target: 5'- aGGuGGUGCAgUCAugacGCG-CCCGCcgACGUAc -3' miRNA: 3'- -CCuCUACGU-AGU----CGCaGGGCG--UGCAU- -5' |
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| 18911 | 3' | -55.2 | NC_004683.1 | + | 5010 | 0.66 | 0.712463 |
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Target: 5'- cGAGgcGCGUCAGCGgaUCCacugcgggCGUGCGUAc -3' miRNA: 3'- cCUCuaCGUAGUCGC--AGG--------GCGUGCAU- -5' |
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| 18911 | 3' | -55.2 | NC_004683.1 | + | 39252 | 0.67 | 0.657903 |
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Target: 5'- gGGGGAUuaagcaggGCAUCAGCaUCCUucaggaGCACGUc -3' miRNA: 3'- -CCUCUA--------CGUAGUCGcAGGG------CGUGCAu -5' |
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| 18911 | 3' | -55.2 | NC_004683.1 | + | 13125 | 0.68 | 0.580769 |
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Target: 5'- cGGAGggGCAUCGGCGggaCCgGUGCc-- -3' miRNA: 3'- -CCUCuaCGUAGUCGCa--GGgCGUGcau -5' |
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| 18911 | 3' | -55.2 | NC_004683.1 | + | 56953 | 0.69 | 0.537428 |
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Target: 5'- gGGGGGUGgGUCGGCGUCgUGaccaGCGa- -3' miRNA: 3'- -CCUCUACgUAGUCGCAGgGCg---UGCau -5' |
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| 18911 | 3' | -55.2 | NC_004683.1 | + | 10631 | 0.69 | 0.505667 |
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Target: 5'- --cGAcUGCAUCAcGCGgaccugCCCGCACGUc -3' miRNA: 3'- ccuCU-ACGUAGU-CGCa-----GGGCGUGCAu -5' |
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| 18911 | 3' | -55.2 | NC_004683.1 | + | 51052 | 0.7 | 0.454654 |
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Target: 5'- --cGAUGCGcCAGCaguUCCCGCAUGUGg -3' miRNA: 3'- ccuCUACGUaGUCGc--AGGGCGUGCAU- -5' |
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| 18911 | 3' | -55.2 | NC_004683.1 | + | 43096 | 0.71 | 0.444785 |
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Target: 5'- cGAGGUGCcgcgugauGUCAGCG-CCgGCGCGg- -3' miRNA: 3'- cCUCUACG--------UAGUCGCaGGgCGUGCau -5' |
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| 18911 | 3' | -55.2 | NC_004683.1 | + | 20305 | 0.71 | 0.413097 |
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Target: 5'- cGGGGcgGCGaCGGCGgcagcgcguucaugUCCCGCGCGa- -3' miRNA: 3'- -CCUCuaCGUaGUCGC--------------AGGGCGUGCau -5' |
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| 18911 | 3' | -55.2 | NC_004683.1 | + | 32470 | 1.09 | 0.000982 |
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Target: 5'- cGGAGAUGCAUCAGCGUCCCGCACGUAu -3' miRNA: 3'- -CCUCUACGUAGUCGCAGGGCGUGCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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