Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18925 | 5' | -55.9 | NC_004683.1 | + | 45939 | 0.66 | 0.712955 |
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Target: 5'- aCACCgaacUGCagggacCGcAGGUuGUCCUCGAGCg -3' miRNA: 3'- aGUGG----ACGa-----GC-UCCAcCAGGAGCUUG- -5' |
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| 18925 | 5' | -55.9 | NC_004683.1 | + | 3129 | 0.66 | 0.702253 |
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Target: 5'- gCGCCgaUGC-CGAuGUGGUCggCUCGAACc -3' miRNA: 3'- aGUGG--ACGaGCUcCACCAG--GAGCUUG- -5' |
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| 18925 | 5' | -55.9 | NC_004683.1 | + | 28136 | 0.66 | 0.702253 |
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Target: 5'- cUC-CCUGCUCGucGgcgaCCUCGAACa -3' miRNA: 3'- -AGuGGACGAGCucCaccaGGAGCUUG- -5' |
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| 18925 | 5' | -55.9 | NC_004683.1 | + | 32918 | 0.67 | 0.604169 |
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Target: 5'- cCACCUcGCUCucGGUGGgggaUCGGGCg -3' miRNA: 3'- aGUGGA-CGAGcuCCACCagg-AGCUUG- -5' |
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| 18925 | 5' | -55.9 | NC_004683.1 | + | 6431 | 0.67 | 0.593262 |
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Target: 5'- gCACCUGUUCGAaccGGUcgaGGUCaaugcCGAGCg -3' miRNA: 3'- aGUGGACGAGCU---CCA---CCAGga---GCUUG- -5' |
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| 18925 | 5' | -55.9 | NC_004683.1 | + | 3425 | 0.7 | 0.46707 |
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Target: 5'- --cCCUGCUCGAuGGUGa-CCUUGGGCg -3' miRNA: 3'- aguGGACGAGCU-CCACcaGGAGCUUG- -5' |
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| 18925 | 5' | -55.9 | NC_004683.1 | + | 39007 | 0.71 | 0.373323 |
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Target: 5'- cUACCUGCUCGAGacggaguccgGGUCCaUCGAcaACg -3' miRNA: 3'- aGUGGACGAGCUCca--------CCAGG-AGCU--UG- -5' |
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| 18925 | 5' | -55.9 | NC_004683.1 | + | 32180 | 0.73 | 0.292468 |
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Target: 5'- gUCGCCgaagaggUGCUCGAGcgcGUCCUCGAACc -3' miRNA: 3'- -AGUGG-------ACGAGCUCcacCAGGAGCUUG- -5' |
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| 18925 | 5' | -55.9 | NC_004683.1 | + | 40688 | 1.09 | 0.0009 |
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Target: 5'- gUCACCUGCUCGAGGUGGUCCUCGAACc -3' miRNA: 3'- -AGUGGACGAGCUCCACCAGGAGCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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