Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18933 | 5' | -53.1 | NC_004683.1 | + | 7390 | 0.66 | 0.84794 |
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Target: 5'- gGCGcUCGACCUGgauUCGgCGGccGGGCGc- -3' miRNA: 3'- gUGC-AGCUGGACa--AGU-GCU--UCCGCau -5' |
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| 18933 | 5' | -53.1 | NC_004683.1 | + | 30766 | 0.66 | 0.839139 |
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Target: 5'- gGCGuucUCGACCUGgcgggcgagCGCGuuGGUGUGg -3' miRNA: 3'- gUGC---AGCUGGACaa-------GUGCuuCCGCAU- -5' |
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| 18933 | 5' | -53.1 | NC_004683.1 | + | 33304 | 0.66 | 0.820876 |
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Target: 5'- gCACGUaggugccuugugCGACCUGgcCGCGGaucuGGGCGa- -3' miRNA: 3'- -GUGCA------------GCUGGACaaGUGCU----UCCGCau -5' |
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| 18933 | 5' | -53.1 | NC_004683.1 | + | 32688 | 0.66 | 0.820876 |
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Target: 5'- -cCGUCGACCgGgccCAUGAGcgcGGCGUAg -3' miRNA: 3'- guGCAGCUGGaCaa-GUGCUU---CCGCAU- -5' |
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| 18933 | 5' | -53.1 | NC_004683.1 | + | 40070 | 0.69 | 0.664161 |
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Target: 5'- aCGCGUCGACgcgCUGUUCGCccGGGCc-- -3' miRNA: 3'- -GUGCAGCUG---GACAAGUGcuUCCGcau -5' |
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| 18933 | 5' | -53.1 | NC_004683.1 | + | 30740 | 0.69 | 0.653034 |
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Target: 5'- uCGCGcUCGACCUcUUCGCGuucGGCGg- -3' miRNA: 3'- -GUGC-AGCUGGAcAAGUGCuu-CCGCau -5' |
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| 18933 | 5' | -53.1 | NC_004683.1 | + | 17535 | 0.69 | 0.641887 |
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Target: 5'- gGCGagGuCCUGgcCGCGAGGGCGa- -3' miRNA: 3'- gUGCagCuGGACaaGUGCUUCCGCau -5' |
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| 18933 | 5' | -53.1 | NC_004683.1 | + | 48068 | 0.7 | 0.619576 |
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Target: 5'- aAUGUcCGACCUGUUCcACGAccAGGUGc- -3' miRNA: 3'- gUGCA-GCUGGACAAG-UGCU--UCCGCau -5' |
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| 18933 | 5' | -53.1 | NC_004683.1 | + | 8665 | 0.71 | 0.531518 |
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Target: 5'- gCGCGaCGACCUGUUCGCucacaagcucGAAagcGGCGUGu -3' miRNA: 3'- -GUGCaGCUGGACAAGUG----------CUU---CCGCAU- -5' |
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| 18933 | 5' | -53.1 | NC_004683.1 | + | 43004 | 1.06 | 0.00277 |
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Target: 5'- uCACGUCGACCUGUUCACGAAGGCGUAc -3' miRNA: 3'- -GUGCAGCUGGACAAGUGCUUCCGCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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