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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18943 | 3' | -48.4 | NC_004683.1 | + | 39514 | 0.66 | 0.957074 |
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Target: 5'- cGAGGUcc-UGGCCGC-GAgGGCGAAu -3' miRNA: 3'- uUUCCAaauACUGGCGaCUgUCGUUU- -5' |
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| 18943 | 3' | -48.4 | NC_004683.1 | + | 25737 | 0.66 | 0.947703 |
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Target: 5'- cGGGGUUUGggcagGACCGCcaccgcgaucGGCGGCAGc -3' miRNA: 3'- uUUCCAAAUa----CUGGCGa---------CUGUCGUUu -5' |
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| 18943 | 3' | -48.4 | NC_004683.1 | + | 47351 | 0.67 | 0.93127 |
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Target: 5'- --cGGUcgGUGGCCGCgacaagGGCAuGCAGGu -3' miRNA: 3'- uuuCCAaaUACUGGCGa-----CUGU-CGUUU- -5' |
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| 18943 | 3' | -48.4 | NC_004683.1 | + | 21705 | 0.67 | 0.93127 |
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Target: 5'- --cGGUgcggGUGG-CGCUGGCAGCGc- -3' miRNA: 3'- uuuCCAaa--UACUgGCGACUGUCGUuu -5' |
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| 18943 | 3' | -48.4 | NC_004683.1 | + | 46600 | 0.67 | 0.925148 |
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Target: 5'- --cGGUcgagGGCCGC-GACGGCAAGg -3' miRNA: 3'- uuuCCAaauaCUGGCGaCUGUCGUUU- -5' |
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| 18943 | 3' | -48.4 | NC_004683.1 | + | 26245 | 0.67 | 0.911931 |
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Target: 5'- --cGGUUcAUGAUCGCgagGAuCAGCGAGa -3' miRNA: 3'- uuuCCAAaUACUGGCGa--CU-GUCGUUU- -5' |
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| 18943 | 3' | -48.4 | NC_004683.1 | + | 45341 | 1.04 | 0.007835 |
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Target: 5'- cAAAGGUUUAUGACCGCUGACAGCAAAu -3' miRNA: 3'- -UUUCCAAAUACUGGCGACUGUCGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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