Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18948 | 5' | -57.1 | NC_004683.1 | + | 24062 | 0.69 | 0.455918 |
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Target: 5'- gGCGUGGUGAUCgcgccgAGGGGCAcguagCCGu -3' miRNA: 3'- -CGCACCGCUAG------UCCCCGUcuacaGGU- -5' |
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| 18948 | 5' | -57.1 | NC_004683.1 | + | 37061 | 0.69 | 0.427078 |
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Target: 5'- gGCGgGGCGGuUCGGGcGGC-GAUGcCCAg -3' miRNA: 3'- -CGCaCCGCU-AGUCC-CCGuCUACaGGU- -5' |
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| 18948 | 5' | -57.1 | NC_004683.1 | + | 42581 | 0.7 | 0.364216 |
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Target: 5'- cGCGccgcGGCGGUC-GGGGCcGGUcGUCCGg -3' miRNA: 3'- -CGCa---CCGCUAGuCCCCGuCUA-CAGGU- -5' |
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| 18948 | 5' | -57.1 | NC_004683.1 | + | 45470 | 0.73 | 0.265378 |
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Target: 5'- --uUGGCGGUCAGGGGUGGccGUGaCCAc -3' miRNA: 3'- cgcACCGCUAGUCCCCGUC--UACaGGU- -5' |
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| 18948 | 5' | -57.1 | NC_004683.1 | + | 42302 | 0.74 | 0.216084 |
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Target: 5'- ---cGGCucaccgGAUCcGGGGCAGAUGUCCGc -3' miRNA: 3'- cgcaCCG------CUAGuCCCCGUCUACAGGU- -5' |
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| 18948 | 5' | -57.1 | NC_004683.1 | + | 46980 | 1.1 | 0.000552 |
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Target: 5'- cGCGUGGCGAUCAGGGGCAGAUGUCCAc -3' miRNA: 3'- -CGCACCGCUAGUCCCCGUCUACAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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