Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18951 | 3' | -59.5 | NC_004683.1 | + | 10126 | 0.66 | 0.468149 |
Target: 5'- --cGCCGCgGCCGAAC----GGCUCGg -3' miRNA: 3'- uacCGGCGaCGGCUUGcacaCCGAGC- -5' |
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18951 | 3' | -59.5 | NC_004683.1 | + | 15267 | 0.66 | 0.478044 |
Target: 5'- -gGGCCGCUGCUGcccAACcagGUGGUcuacaUCGa -3' miRNA: 3'- uaCCGGCGACGGC---UUGca-CACCG-----AGC- -5' |
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18951 | 3' | -59.5 | NC_004683.1 | + | 25515 | 0.66 | 0.488041 |
Target: 5'- cGUGGUugaaGCUgacGCCGugcCGcUGUGGCUCGg -3' miRNA: 3'- -UACCGg---CGA---CGGCuu-GC-ACACCGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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