miRNA: 3'- -UCCAGUUGgUUUg---GGCUCAAGaCCGc -5'
miRNA: 3'- -UCCAGUUGGuu--------UGGGCUCaaGaCCGC- -5'
miRNA: 3'- -UC-CAGU-------UGGUUUGGGCUCa--AGACC-GC- -5'
miRNA: 3'- uCCAGUUGGUU--------UGGGCUCAaGACcGC- -5'
miRNA: 3'- -UCCAGUUGGUUUGGGCUCAAGACCGC- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18957 | 5' | -52.9 | NC_004683.1 | + | 15242 | 0.71 | 0.540359 |
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Target: 5'- cGGGUCGACgGAGaugaUCGAGUUCgGGCc -3' miRNA: 3'- -UCCAGUUGgUUUg---GGCUCAAGaCCGc -5' |
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| 18957 | 5' | -52.9 | NC_004683.1 | + | 3942 | 0.72 | 0.518828 |
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Target: 5'- gAGGUCGACCccgugcucgcACCCGAGggC-GGCGc -3' miRNA: 3'- -UCCAGUUGGuu--------UGGGCUCaaGaCCGC- -5' |
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| 18957 | 5' | -52.9 | NC_004683.1 | + | 55867 | 0.73 | 0.445518 |
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Target: 5'- uGGuGUCGuguuuguGCCAGACCCGAGcgaUCUGGuCGg -3' miRNA: 3'- -UC-CAGU-------UGGUUUGGGCUCa--AGACC-GC- -5' |
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| 18957 | 5' | -52.9 | NC_004683.1 | + | 52593 | 0.73 | 0.426889 |
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Target: 5'- uGGUCAACCGGcccgagccACCCGAGUaCUG-CGg -3' miRNA: 3'- uCCAGUUGGUU--------UGGGCUCAaGACcGC- -5' |
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| 18957 | 5' | -52.9 | NC_004683.1 | + | 50463 | 1.08 | 0.002026 |
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Target: 5'- cAGGUCAACCAAACCCGAGUUCUGGCGc -3' miRNA: 3'- -UCCAGUUGGUUUGGGCUCAAGACCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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