Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18961 | 3' | -56.7 | NC_004683.1 | + | 4586 | 0.7 | 0.402442 |
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Target: 5'- --aACCUGCGcGGU-GUCGACGGGUGc -3' miRNA: 3'- ucgUGGAUGCaCUAcCAGCUGCCCGC- -5' |
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| 18961 | 3' | -56.7 | NC_004683.1 | + | 13987 | 0.71 | 0.393457 |
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Target: 5'- cGGCACCcgUugGUGAcgcugUGGUCcuacGACgGGGCGa -3' miRNA: 3'- -UCGUGG--AugCACU-----ACCAG----CUG-CCCGC- -5' |
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| 18961 | 3' | -56.7 | NC_004683.1 | + | 46516 | 0.72 | 0.342354 |
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Target: 5'- cGGCuccaagACCUgguuGCGgcacGUGGUCGACGGGCa -3' miRNA: 3'- -UCG------UGGA----UGCac--UACCAGCUGCCCGc -5' |
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| 18961 | 3' | -56.7 | NC_004683.1 | + | 44684 | 0.72 | 0.334317 |
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Target: 5'- cAGCcuucGCCUcgucgaACGUGA-GGUCGGCGGcGCGc -3' miRNA: 3'- -UCG----UGGA------UGCACUaCCAGCUGCC-CGC- -5' |
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| 18961 | 3' | -56.7 | NC_004683.1 | + | 32675 | 0.73 | 0.303559 |
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Target: 5'- gAGCGgCUACGUGGUGGUgaACGaGGCu -3' miRNA: 3'- -UCGUgGAUGCACUACCAgcUGC-CCGc -5' |
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| 18961 | 3' | -56.7 | NC_004683.1 | + | 29694 | 0.73 | 0.275023 |
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Target: 5'- aGGCGCCggcUACGcuugGAUGGUCGACGaaaGCGg -3' miRNA: 3'- -UCGUGG---AUGCa---CUACCAGCUGCc--CGC- -5' |
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| 18961 | 3' | -56.7 | NC_004683.1 | + | 41247 | 0.74 | 0.236302 |
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Target: 5'- uGCGCCUGCGUGAcUGGcUG-UGGGCGc -3' miRNA: 3'- uCGUGGAUGCACU-ACCaGCuGCCCGC- -5' |
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| 18961 | 3' | -56.7 | NC_004683.1 | + | 44398 | 0.76 | 0.197014 |
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Target: 5'- gAGCGCCUGCGacugUGAcaGGUCGGugccCGGGCGa -3' miRNA: 3'- -UCGUGGAUGC----ACUa-CCAGCU----GCCCGC- -5' |
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| 18961 | 3' | -56.7 | NC_004683.1 | + | 51917 | 1.08 | 0.001008 |
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Target: 5'- cAGCACCUACGUGAUGGUCGACGGGCGc -3' miRNA: 3'- -UCGUGGAUGCACUACCAGCUGCCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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