Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18963 | 5' | -52.7 | NC_004683.1 | + | 3489 | 0.68 | 0.809193 |
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Target: 5'- cGGCGgGCuACGAGC-CGgaaucgGugGUGGc -3' miRNA: 3'- cCCGUgCGuUGCUUGaGCa-----CugCACC- -5' |
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| 18963 | 5' | -52.7 | NC_004683.1 | + | 2756 | 0.73 | 0.491798 |
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Target: 5'- cGGC-CGCGGCGAGCUCGgcGGCGg-- -3' miRNA: 3'- cCCGuGCGUUGCUUGAGCa-CUGCacc -5' |
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| 18963 | 5' | -52.7 | NC_004683.1 | + | 2351 | 0.69 | 0.728771 |
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Target: 5'- cGGCAUcgGCuACGggUUCG-GugGUGGc -3' miRNA: 3'- cCCGUG--CGuUGCuuGAGCaCugCACC- -5' |
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| 18963 | 5' | -52.7 | NC_004683.1 | + | 1210 | 0.66 | 0.865336 |
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Target: 5'- cGGcCGCGCuuGACGAGCUUgugcagcaccucggaGUcGGCGUGGc -3' miRNA: 3'- cCC-GUGCG--UUGCUUGAG---------------CA-CUGCACC- -5' |
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| 18963 | 5' | -52.7 | NC_004683.1 | + | 318 | 0.69 | 0.739279 |
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Target: 5'- cGGGCA-GCAGCGGcggcaucgcggcGCUCG-GA-GUGGg -3' miRNA: 3'- -CCCGUgCGUUGCU------------UGAGCaCUgCACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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