miRNA: 3'- cCCGuGCGUUGCu---UGAgCACugC-ACC- -5'
miRNA: 3'- -CCCGU-----GCGUUGCuUGA-GCACUGCa-----CC- -5'
miRNA: 3'- -CCCGug-CGU---UGCUUGaGCACUG-CACC- -5'
miRNA: 3'- cCCGUgCGuUGCUUGaGCa-----CugCACC- -5'
miRNA: 3'- -CCCGUGCGUUGCUUGAGCACUGCACC- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18963 | 5' | -52.7 | NC_004683.1 | + | 6671 | 0.66 | 0.870125 |
|
Target: 5'- uGGC-CGCGGCGcgccGCUgGUGugGcUGGc -3' miRNA: 3'- cCCGuGCGUUGCu---UGAgCACugC-ACC- -5' |
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| 18963 | 5' | -52.7 | NC_004683.1 | + | 47572 | 0.67 | 0.845326 |
|
Target: 5'- gGGGCGuccccUGCGACG-GCUgCGUGGCGgcguucGGg -3' miRNA: 3'- -CCCGU-----GCGUUGCuUGA-GCACUGCa-----CC- -5' |
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| 18963 | 5' | -52.7 | NC_004683.1 | + | 31296 | 0.67 | 0.845326 |
|
Target: 5'- gGGGUuguGCAgcuGCGGGC-CGUGGCgGUGGc -3' miRNA: 3'- -CCCGug-CGU---UGCUUGaGCACUG-CACC- -5' |
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| 18963 | 5' | -52.7 | NC_004683.1 | + | 3489 | 0.68 | 0.809193 |
|
Target: 5'- cGGCGgGCuACGAGC-CGgaaucgGugGUGGc -3' miRNA: 3'- cCCGUgCGuUGCUUGaGCa-----CugCACC- -5' |
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| 18963 | 5' | -52.7 | NC_004683.1 | + | 52403 | 1.14 | 0.001076 |
|
Target: 5'- aGGGCACGCAACGAACUCGUGACGUGGg -3' miRNA: 3'- -CCCGUGCGUUGCUUGAGCACUGCACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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