Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18964 | 5' | -54.9 | NC_004683.1 | + | 5347 | 0.71 | 0.458993 |
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Target: 5'- gCGUGCCaucuucgaccaGGCGCGcagGUaCGCCGGCGAc -3' miRNA: 3'- -GCACGG-----------UCGUGCa--CAaGUGGCUGCUc -5' |
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| 18964 | 5' | -54.9 | NC_004683.1 | + | 6691 | 0.71 | 0.458993 |
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Target: 5'- uGUgGCUGGCACGUGUcgcccgugaUCAUCGAUGAc -3' miRNA: 3'- gCA-CGGUCGUGCACA---------AGUGGCUGCUc -5' |
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| 18964 | 5' | -54.9 | NC_004683.1 | + | 44131 | 0.72 | 0.411054 |
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Target: 5'- --cGCUguGGgACGUGUUCaccGCCGACGAGa -3' miRNA: 3'- gcaCGG--UCgUGCACAAG---UGGCUGCUC- -5' |
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| 18964 | 5' | -54.9 | NC_004683.1 | + | 20047 | 0.77 | 0.194975 |
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Target: 5'- gGUGauaCGGCGCGguUGggCACCGACGAGg -3' miRNA: 3'- gCACg--GUCGUGC--ACaaGUGGCUGCUC- -5' |
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| 18964 | 5' | -54.9 | NC_004683.1 | + | 40470 | 0.81 | 0.112751 |
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Target: 5'- aGUGUCGGCGCGUGcaCACCGcCGAGg -3' miRNA: 3'- gCACGGUCGUGCACaaGUGGCuGCUC- -5' |
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| 18964 | 5' | -54.9 | NC_004683.1 | + | 52628 | 1.1 | 0.001087 |
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Target: 5'- cCGUGCCAGCACGUGUUCACCGACGAGg -3' miRNA: 3'- -GCACGGUCGUGCACAAGUGGCUGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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