Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18965 | 5' | -51.9 | NC_004683.1 | + | 2755 | 0.66 | 0.860972 |
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Target: 5'- --gCGGCcgCGGCGAgCUcGGCGGCg -3' miRNA: 3'- caaGUCGuaGUCGCUaGAcUCGCUGa -5' |
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| 18965 | 5' | -51.9 | NC_004683.1 | + | 54132 | 0.66 | 0.860972 |
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Target: 5'- -gUUGGCGUCggAGCGGagCUGGGCGAg- -3' miRNA: 3'- caAGUCGUAG--UCGCUa-GACUCGCUga -5' |
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| 18965 | 5' | -51.9 | NC_004683.1 | + | 5851 | 0.67 | 0.815508 |
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Target: 5'- -cUCGGCGgugacuggAGCGAUCUcGGCGGCg -3' miRNA: 3'- caAGUCGUag------UCGCUAGAcUCGCUGa -5' |
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| 18965 | 5' | -51.9 | NC_004683.1 | + | 3595 | 0.68 | 0.785675 |
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Target: 5'- -gUCcGCGUCGGCG-UC-GAGCGAUa -3' miRNA: 3'- caAGuCGUAGUCGCuAGaCUCGCUGa -5' |
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| 18965 | 5' | -51.9 | NC_004683.1 | + | 32903 | 0.68 | 0.743565 |
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Target: 5'- -cUUGGCcgagAUCAGCGGUCUGcgaugguGCGACg -3' miRNA: 3'- caAGUCG----UAGUCGCUAGACu------CGCUGa -5' |
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| 18965 | 5' | -51.9 | NC_004683.1 | + | 15096 | 0.69 | 0.721735 |
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Target: 5'- --cCAGCAUCGGUgcgcaaGAUCgccgcGGGCGACa -3' miRNA: 3'- caaGUCGUAGUCG------CUAGa----CUCGCUGa -5' |
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| 18965 | 5' | -51.9 | NC_004683.1 | + | 51518 | 0.69 | 0.720633 |
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Target: 5'- -aUCGGCAUCGGCGAcCUGAuccaucacgcacgGCaGGCg -3' miRNA: 3'- caAGUCGUAGUCGCUaGACU-------------CG-CUGa -5' |
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| 18965 | 5' | -51.9 | NC_004683.1 | + | 56296 | 0.7 | 0.665713 |
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Target: 5'- --cCAGCGUCGGgGAUaCUG-GCGGCc -3' miRNA: 3'- caaGUCGUAGUCgCUA-GACuCGCUGa -5' |
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| 18965 | 5' | -51.9 | NC_004683.1 | + | 52818 | 1.08 | 0.002384 |
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Target: 5'- cGUUCAGCAUCAGCGAUCUGAGCGACUu -3' miRNA: 3'- -CAAGUCGUAGUCGCUAGACUCGCUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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