Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18967 | 5' | -55.2 | NC_004683.1 | + | 49274 | 0.67 | 0.697345 |
Target: 5'- uGCCCGaCGACGUC--GCGG-CACa--- -3' miRNA: 3'- -CGGGC-GCUGCAGugUGCCaGUGaagu -5' |
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18967 | 5' | -55.2 | NC_004683.1 | + | 51297 | 0.66 | 0.770101 |
Target: 5'- aGCCCGUucGACGcCACAgGGgCGC-UCGc -3' miRNA: 3'- -CGGGCG--CUGCaGUGUgCCaGUGaAGU- -5' |
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18967 | 5' | -55.2 | NC_004683.1 | + | 53212 | 0.67 | 0.697345 |
Target: 5'- --gCGCGaACGUCgACAUGGUCACcUCc -3' miRNA: 3'- cggGCGC-UGCAG-UGUGCCAGUGaAGu -5' |
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18967 | 5' | -55.2 | NC_004683.1 | + | 54553 | 1.11 | 0.00093 |
Target: 5'- cGCCCGCGACGUCACACGGUCACUUCAu -3' miRNA: 3'- -CGGGCGCUGCAGUGUGCCAGUGAAGU- -5' |
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18967 | 5' | -55.2 | NC_004683.1 | + | 54903 | 0.66 | 0.767096 |
Target: 5'- gGCUCaaCGACGUCACcaaggucuacgacgACGGUCgccACUUCGc -3' miRNA: 3'- -CGGGc-GCUGCAGUG--------------UGCCAG---UGAAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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