miRNA: 3'- aGGUGGCCGC-CGuccuauACGUCCGC- -5'
miRNA: 3'- agGUGGCCG--CCGuCCuaUACGUC-CGc -5'
miRNA: 3'- -AGGugGCCGc-CguCCUAUACGU----CCGC- -5'
miRNA: 3'- -AGGUGGCCGCCG--------------UCCUAuacGUCCGC- -5'
Results 101 - 104 of 104 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18989 | 3' | -59.3 | NC_004684.1 | + | 2957 | 0.66 | 0.53441 |
|
Target: 5'- gCCAUCGGCGcGCugcuguUGCGGGUGg -3' miRNA: 3'- aGGUGGCCGC-CGuccuauACGUCCGC- -5' |
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| 18989 | 3' | -59.3 | NC_004684.1 | + | 2784 | 0.66 | 0.53441 |
|
Target: 5'- -gCACCGGCcaGGCcGGggAUGCGGuGCc -3' miRNA: 3'- agGUGGCCG--CCGuCCuaUACGUC-CGc -5' |
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| 18989 | 3' | -59.3 | NC_004684.1 | + | 1955 | 0.66 | 0.514008 |
|
Target: 5'- aUCCuuCGGCcaGguGGAUAUGCGgcccGGCGu -3' miRNA: 3'- -AGGugGCCGc-CguCCUAUACGU----CCGC- -5' |
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| 18989 | 3' | -59.3 | NC_004684.1 | + | 785 | 0.77 | 0.115697 |
|
Target: 5'- gUCCACCGGCGGCugaccucggggccgAGGAU---CAGGCGc -3' miRNA: 3'- -AGGUGGCCGCCG--------------UCCUAuacGUCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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