Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18991 | 5' | -57.9 | NC_004684.1 | + | 64992 | 0.66 | 0.56637 |
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Target: 5'- gGUCUGCggcucgCCaGCgGCGUUGGUGGuGUGg -3' miRNA: 3'- -CAGACGa-----GGaCGgUGUAGUCGCC-CAC- -5' |
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| 18991 | 5' | -57.9 | NC_004684.1 | + | 16691 | 0.67 | 0.545163 |
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Target: 5'- uGUCUGg-CCUGCCGCcu-GGCGuGGUGu -3' miRNA: 3'- -CAGACgaGGACGGUGuagUCGC-CCAC- -5' |
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| 18991 | 5' | -57.9 | NC_004684.1 | + | 18528 | 0.67 | 0.503588 |
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Target: 5'- -cCUGCUCgggaGCCACGUCGGCcgccGGUGc -3' miRNA: 3'- caGACGAGga--CGGUGUAGUCGc---CCAC- -5' |
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| 18991 | 5' | -57.9 | NC_004684.1 | + | 61164 | 0.68 | 0.493406 |
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Target: 5'- gGUCaGCcacCCgGCCAgGUCGGCGGGg- -3' miRNA: 3'- -CAGaCGa--GGaCGGUgUAGUCGCCCac -5' |
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| 18991 | 5' | -57.9 | NC_004684.1 | + | 25456 | 0.69 | 0.434473 |
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Target: 5'- -aCUGCUCCggUGCCA--UCAGCGaccuGGUGg -3' miRNA: 3'- caGACGAGG--ACGGUguAGUCGC----CCAC- -5' |
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| 18991 | 5' | -57.9 | NC_004684.1 | + | 44750 | 0.69 | 0.388612 |
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Target: 5'- ---cGCgCC-GCCGCAgCAGCGGGUGa -3' miRNA: 3'- cagaCGaGGaCGGUGUaGUCGCCCAC- -5' |
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| 18991 | 5' | -57.9 | NC_004684.1 | + | 53095 | 0.7 | 0.337942 |
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Target: 5'- -cCUGCgcggcggCCUgcaagGCCACAUCAcGCGGGUa -3' miRNA: 3'- caGACGa------GGA-----CGGUGUAGU-CGCCCAc -5' |
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| 18991 | 5' | -57.9 | NC_004684.1 | + | 13055 | 0.7 | 0.337942 |
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Target: 5'- -gUUGCcugCC-GCCGCGUCGGCGGGa- -3' miRNA: 3'- caGACGa--GGaCGGUGUAGUCGCCCac -5' |
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| 18991 | 5' | -57.9 | NC_004684.1 | + | 18896 | 0.76 | 0.156848 |
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Target: 5'- ---gGCUCCU-CCACGUCGGCGGGg- -3' miRNA: 3'- cagaCGAGGAcGGUGUAGUCGCCCac -5' |
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| 18991 | 5' | -57.9 | NC_004684.1 | + | 11079 | 1.07 | 0.000855 |
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Target: 5'- gGUCUGCUCCUGCCACAUCAGCGGGUGc -3' miRNA: 3'- -CAGACGAGGACGGUGUAGUCGCCCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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