miRNA: 3'- -CGGUaacua-GACCGa-CCA-GCGGGCGa -5'
miRNA: 3'- -CGGUaa--CUA-GACCGACCAgCGGgC-GA- -5'
miRNA: 3'- -CGGUa--ACUaGACCGa--CCAGCGGGCgA- -5'
miRNA: 3'- -CGGUAACUAGACCGACCAGCGGGCGA- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18993 | 5' | -57 | NC_004684.1 | + | 44080 | 0.71 | 0.36767 |
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Target: 5'- gGCCAacgcagCUGGCcgGGUaCGCCCGCg -3' miRNA: 3'- -CGGUaacua-GACCGa-CCA-GCGGGCGa -5' |
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| 18993 | 5' | -57 | NC_004684.1 | + | 57879 | 0.71 | 0.36767 |
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Target: 5'- cGCCAgaccGGUgCUGGCUGGUgGCCgGgCUg -3' miRNA: 3'- -CGGUaa--CUA-GACCGACCAgCGGgC-GA- -5' |
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| 18993 | 5' | -57 | NC_004684.1 | + | 4054 | 0.71 | 0.350925 |
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Target: 5'- gGCCAggaUGAcCUGGCcggGGUCGUCCGgUg -3' miRNA: 3'- -CGGUa--ACUaGACCGa--CCAGCGGGCgA- -5' |
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| 18993 | 5' | -57 | NC_004684.1 | + | 13308 | 1.09 | 0.000784 |
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Target: 5'- cGCCAUUGAUCUGGCUGGUCGCCCGCUc -3' miRNA: 3'- -CGGUAACUAGACCGACCAGCGGGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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