Results 101 - 108 of 108 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18995 | 5' | -61.1 | NC_004684.1 | + | 21376 | 0.66 | 0.470199 |
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Target: 5'- uUGCGCagauacGGCcgGGCGcGCGUGC-CGGGGu -3' miRNA: 3'- cACGCG------CCG--CCGCuUGCACGcGUCCU- -5' |
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| 18995 | 5' | -61.1 | NC_004684.1 | + | 13022 | 0.66 | 0.470199 |
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Target: 5'- gGUGcCGaCGGCGGCGAaguuggccgACG-GCGCAccGGc -3' miRNA: 3'- -CAC-GC-GCCGCCGCU---------UGCaCGCGU--CCu -5' |
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| 18995 | 5' | -61.1 | NC_004684.1 | + | 59700 | 0.66 | 0.470199 |
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Target: 5'- cUGCGCGG-GGUGcGgGUGCGCAu-- -3' miRNA: 3'- cACGCGCCgCCGCuUgCACGCGUccu -5' |
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| 18995 | 5' | -61.1 | NC_004684.1 | + | 16749 | 0.66 | 0.479837 |
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Target: 5'- -aGCGCGGCuGGCcugGAACu--CGCGGGGa -3' miRNA: 3'- caCGCGCCG-CCG---CUUGcacGCGUCCU- -5' |
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| 18995 | 5' | -61.1 | NC_004684.1 | + | 25563 | 0.66 | 0.479837 |
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Target: 5'- -gGCGCGGUGGa--AgGUGCGCuacccGGAg -3' miRNA: 3'- caCGCGCCGCCgcuUgCACGCGu----CCU- -5' |
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| 18995 | 5' | -61.1 | NC_004684.1 | + | 60067 | 0.66 | 0.479837 |
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Target: 5'- -cGCGCGGCccGGCG-GCccGUGCGGGc -3' miRNA: 3'- caCGCGCCG--CCGCuUGcaCGCGUCCu -5' |
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| 18995 | 5' | -61.1 | NC_004684.1 | + | 27169 | 0.66 | 0.479837 |
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Target: 5'- cUGgGCGGCGauguccacgccuGCGGACccgGCGCuGGAg -3' miRNA: 3'- cACgCGCCGC------------CGCUUGca-CGCGuCCU- -5' |
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| 18995 | 5' | -61.1 | NC_004684.1 | + | 23714 | 0.65 | 0.486641 |
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Target: 5'- cUGgGCGGCGGCGcgcucaacaucgcuGGCG-GCGCAc-- -3' miRNA: 3'- cACgCGCCGCCGC--------------UUGCaCGCGUccu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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