Results 101 - 108 of 108 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18995 | 5' | -61.1 | NC_004684.1 | + | 12018 | 0.69 | 0.323877 |
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Target: 5'- --uCGCGGCGGCGGugGccaagGCcaugcucgaccaGCAGGAg -3' miRNA: 3'- cacGCGCCGCCGCUugCa----CG------------CGUCCU- -5' |
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| 18995 | 5' | -61.1 | NC_004684.1 | + | 46739 | 0.68 | 0.329186 |
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Target: 5'- uUGagGCGGCGGUauuguccagcgugcGGACGcaggcgGCGCAGGAa -3' miRNA: 3'- cACg-CGCCGCCG--------------CUUGCa-----CGCGUCCU- -5' |
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| 18995 | 5' | -61.1 | NC_004684.1 | + | 59456 | 0.68 | 0.339216 |
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Target: 5'- uGUGCGCcggucGGCGGCGAuguccaccACcUGCGCGGc- -3' miRNA: 3'- -CACGCG-----CCGCCGCU--------UGcACGCGUCcu -5' |
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| 18995 | 5' | -61.1 | NC_004684.1 | + | 66529 | 0.68 | 0.342347 |
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Target: 5'- -aGCGCGGCccGGCGGGCacggcgguaggcguuGUGCGCcucaccGGAa -3' miRNA: 3'- caCGCGCCG--CCGCUUG---------------CACGCGu-----CCU- -5' |
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| 18995 | 5' | -61.1 | NC_004684.1 | + | 20120 | 0.68 | 0.347082 |
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Target: 5'- cUGgGCGGCGGCaccaaGAACGUGCGg---- -3' miRNA: 3'- cACgCGCCGCCG-----CUUGCACGCguccu -5' |
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| 18995 | 5' | -61.1 | NC_004684.1 | + | 59120 | 0.68 | 0.347082 |
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Target: 5'- -aGCGCcgcaggucGCGGCGcACGUG-GCGGGAc -3' miRNA: 3'- caCGCGc-------CGCCGCuUGCACgCGUCCU- -5' |
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| 18995 | 5' | -61.1 | NC_004684.1 | + | 6161 | 0.68 | 0.355077 |
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Target: 5'- ---gGCGGCGGCcAGCGUGCgGCGGu- -3' miRNA: 3'- cacgCGCCGCCGcUUGCACG-CGUCcu -5' |
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| 18995 | 5' | -61.1 | NC_004684.1 | + | 48732 | 0.67 | 0.423574 |
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Target: 5'- uUGC-CGGUGGCG-AUGUG-GCAGGc -3' miRNA: 3'- cACGcGCCGCCGCuUGCACgCGUCCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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