Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18996 | 5' | -56.7 | NC_004684.1 | + | 6795 | 0.72 | 0.377582 |
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Target: 5'- uCGGCuuGCUCgcgCCgacGGUGGCCAGGUCGa -3' miRNA: 3'- -GCUG--UGGGa--GGac-UUACCGGUCCAGC- -5' |
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| 18996 | 5' | -56.7 | NC_004684.1 | + | 33544 | 0.72 | 0.377582 |
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Target: 5'- aCGGCACCgUCCUGuugaGGCgCGGcGUCGa -3' miRNA: 3'- -GCUGUGGgAGGACuua-CCG-GUC-CAGC- -5' |
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| 18996 | 5' | -56.7 | NC_004684.1 | + | 11888 | 0.74 | 0.277231 |
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Target: 5'- -cGCGCCCUUCUccacGGUGGCCuGGUCGg -3' miRNA: 3'- gcUGUGGGAGGAc---UUACCGGuCCAGC- -5' |
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| 18996 | 5' | -56.7 | NC_004684.1 | + | 25066 | 0.74 | 0.257325 |
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Target: 5'- gGAUGCCgC-CCUGGgacaccggaagcGUGGCCGGGUCGg -3' miRNA: 3'- gCUGUGG-GaGGACU------------UACCGGUCCAGC- -5' |
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| 18996 | 5' | -56.7 | NC_004684.1 | + | 50114 | 0.74 | 0.257325 |
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Target: 5'- uGGCcCCCaCCUcGAUGGCCAGGUUGa -3' miRNA: 3'- gCUGuGGGaGGAcUUACCGGUCCAGC- -5' |
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| 18996 | 5' | -56.7 | NC_004684.1 | + | 13962 | 1.09 | 0.000929 |
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Target: 5'- uCGACACCCUCCUGAAUGGCCAGGUCGg -3' miRNA: 3'- -GCUGUGGGAGGACUUACCGGUCCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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