Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19003 | 3' | -56.5 | NC_004684.1 | + | 25300 | 0.66 | 0.717101 |
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Target: 5'- --cACCAGGcGGcGGCgGUUCgUCGGCc -3' miRNA: 3'- gcaUGGUCCaCC-CCGaCAAG-AGUCGc -5' |
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| 19003 | 3' | -56.5 | NC_004684.1 | + | 59004 | 0.66 | 0.706598 |
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Target: 5'- cCGgagGCgGGGUGGGGCgGgga-CGGCGa -3' miRNA: 3'- -GCa--UGgUCCACCCCGaCaagaGUCGC- -5' |
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| 19003 | 3' | -56.5 | NC_004684.1 | + | 41446 | 0.67 | 0.663991 |
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Target: 5'- gGUGCCGccggugucaGUGGGGCUGacCcCGGCGg -3' miRNA: 3'- gCAUGGUc--------CACCCCGACaaGaGUCGC- -5' |
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| 19003 | 3' | -56.5 | NC_004684.1 | + | 31926 | 0.67 | 0.631699 |
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Target: 5'- -cUGCC-GGUcGGGGCUcGUUCUCcGUGg -3' miRNA: 3'- gcAUGGuCCA-CCCCGA-CAAGAGuCGC- -5' |
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| 19003 | 3' | -56.5 | NC_004684.1 | + | 18859 | 0.68 | 0.588688 |
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Target: 5'- cCGUACCggccucGGGUacgGGGGUUGUUUcgUCGGUGc -3' miRNA: 3'- -GCAUGG------UCCA---CCCCGACAAG--AGUCGC- -5' |
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| 19003 | 3' | -56.5 | NC_004684.1 | + | 57321 | 0.69 | 0.515036 |
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Target: 5'- ---cCCAGGUGGGGC-GUUUgucCGGCa -3' miRNA: 3'- gcauGGUCCACCCCGaCAAGa--GUCGc -5' |
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| 19003 | 3' | -56.5 | NC_004684.1 | + | 61176 | 0.71 | 0.399009 |
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Target: 5'- --gGCCAGGUcggcGGGGUUGguggcCUCGGCGu -3' miRNA: 3'- gcaUGGUCCA----CCCCGACaa---GAGUCGC- -5' |
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| 19003 | 3' | -56.5 | NC_004684.1 | + | 67071 | 0.75 | 0.240595 |
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Target: 5'- cCGUACCcGGUGcGGGCguUGgcCUCGGCGg -3' miRNA: 3'- -GCAUGGuCCAC-CCCG--ACaaGAGUCGC- -5' |
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| 19003 | 3' | -56.5 | NC_004684.1 | + | 17020 | 1.09 | 0.000909 |
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Target: 5'- cCGUACCAGGUGGGGCUGUUCUCAGCGa -3' miRNA: 3'- -GCAUGGUCCACCCCGACAAGAGUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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