Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19009 | 5' | -55.4 | NC_004684.1 | + | 7553 | 0.72 | 0.429424 |
Target: 5'- gGUGCUggaccGGcCCCGGGUGGCGcUGgugcGGCUg -3' miRNA: 3'- -CACGAa----CC-GGGCCUAUUGC-ACa---CCGG- -5' |
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19009 | 5' | -55.4 | NC_004684.1 | + | 19446 | 0.74 | 0.309819 |
Target: 5'- cGUGCgcccauGCCCGGGcGGCG-GUGGCCc -3' miRNA: 3'- -CACGaac---CGGGCCUaUUGCaCACCGG- -5' |
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19009 | 5' | -55.4 | NC_004684.1 | + | 40417 | 0.75 | 0.287735 |
Target: 5'- -cGCaccGGCCUGGAUGGCGgcguUGGCCa -3' miRNA: 3'- caCGaa-CCGGGCCUAUUGCac--ACCGG- -5' |
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19009 | 5' | -55.4 | NC_004684.1 | + | 46196 | 1.13 | 0.000659 |
Target: 5'- cGUGCUUGGCCCGGAUAACGUGUGGCCg -3' miRNA: 3'- -CACGAACCGGGCCUAUUGCACACCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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