Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1901 | 3' | -50.6 | NC_001347.2 | + | 142235 | 0.66 | 0.998649 |
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Target: 5'- -aCGAuGUGCuCUucuucguggAUGGcuGCGAGGCGCUGg -3' miRNA: 3'- caGUU-CAUG-GA---------UACC--UGCUUCGCGAC- -5' |
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| 1901 | 3' | -50.6 | NC_001347.2 | + | 153808 | 0.66 | 0.998649 |
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Target: 5'- -cCGAGUcGCCUccGGGCGAggugGGUGCUu -3' miRNA: 3'- caGUUCA-UGGAuaCCUGCU----UCGCGAc -5' |
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| 1901 | 3' | -50.6 | NC_001347.2 | + | 36449 | 0.66 | 0.998649 |
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Target: 5'- cGUCGAG-ACCggcGGACGcgGAGCGgaGg -3' miRNA: 3'- -CAGUUCaUGGauaCCUGC--UUCGCgaC- -5' |
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| 1901 | 3' | -50.6 | NC_001347.2 | + | 127574 | 0.66 | 0.998375 |
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Target: 5'- -aCAGcGUGCCcGUGGACGcggccGGCGCg- -3' miRNA: 3'- caGUU-CAUGGaUACCUGCu----UCGCGac -5' |
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| 1901 | 3' | -50.6 | NC_001347.2 | + | 196179 | 0.66 | 0.998056 |
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Target: 5'- cGUCAAGUACC---GGACGcaccGCGCc- -3' miRNA: 3'- -CAGUUCAUGGauaCCUGCuu--CGCGac -5' |
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| 1901 | 3' | -50.6 | NC_001347.2 | + | 102411 | 0.66 | 0.997686 |
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Target: 5'- -gCAGGaACCcAUGGGCGGugcGGCGCg- -3' miRNA: 3'- caGUUCaUGGaUACCUGCU---UCGCGac -5' |
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| 1901 | 3' | -50.6 | NC_001347.2 | + | 7857 | 0.67 | 0.994024 |
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Target: 5'- uGUCAGGUAUCcgcGUGGACGGaaacGGCGgUa -3' miRNA: 3'- -CAGUUCAUGGa--UACCUGCU----UCGCgAc -5' |
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| 1901 | 3' | -50.6 | NC_001347.2 | + | 171501 | 0.69 | 0.983119 |
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Target: 5'- aGUCAAGUACUcgauUAUGGcCGAcAGCGUg- -3' miRNA: 3'- -CAGUUCAUGG----AUACCuGCU-UCGCGac -5' |
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| 1901 | 3' | -50.6 | NC_001347.2 | + | 95683 | 0.7 | 0.973859 |
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Target: 5'- -cCAcuUGCUgcugGUGGACGAGGCGCa- -3' miRNA: 3'- caGUucAUGGa---UACCUGCUUCGCGac -5' |
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| 1901 | 3' | -50.6 | NC_001347.2 | + | 149836 | 0.75 | 0.833814 |
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Target: 5'- cUCGAGUACCUGUauaaGGugGAcucgcAGCGCUu -3' miRNA: 3'- cAGUUCAUGGAUA----CCugCU-----UCGCGAc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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