Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19028 | 5' | -57.9 | NC_004684.1 | + | 13760 | 0.66 | 0.599409 |
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Target: 5'- gUGCUCCAgCGCaUGCGGGU--GUcCGGg -3' miRNA: 3'- aACGAGGU-GUG-ACGCCCGggUAaGCC- -5' |
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| 19028 | 5' | -57.9 | NC_004684.1 | + | 46647 | 0.66 | 0.582271 |
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Target: 5'- -gGCaCCGC-CUGCGGuGCCUccaggcgcuccucggGUUCGGg -3' miRNA: 3'- aaCGaGGUGuGACGCC-CGGG---------------UAAGCC- -5' |
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| 19028 | 5' | -57.9 | NC_004684.1 | + | 26225 | 0.68 | 0.504797 |
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Target: 5'- -cGUUCCACcccGCgcGCGGGUCCGgcgCGGc -3' miRNA: 3'- aaCGAGGUG---UGa-CGCCCGGGUaa-GCC- -5' |
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| 19028 | 5' | -57.9 | NC_004684.1 | + | 62297 | 0.68 | 0.474632 |
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Target: 5'- cUGCUCCAgCGCcgcGCGGGCCUccgCGa -3' miRNA: 3'- aACGAGGU-GUGa--CGCCCGGGuaaGCc -5' |
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| 19028 | 5' | -57.9 | NC_004684.1 | + | 3245 | 0.68 | 0.455031 |
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Target: 5'- -cGCggagaCCACGCUGCuGGCCUGgcCGGa -3' miRNA: 3'- aaCGa----GGUGUGACGcCCGGGUaaGCC- -5' |
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| 19028 | 5' | -57.9 | NC_004684.1 | + | 61779 | 0.7 | 0.3642 |
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Target: 5'- -aGCUCCAgCACcaUGCGcgccagcucGGCCCcgUCGGc -3' miRNA: 3'- aaCGAGGU-GUG--ACGC---------CCGGGuaAGCC- -5' |
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| 19028 | 5' | -57.9 | NC_004684.1 | + | 49643 | 0.71 | 0.31529 |
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Target: 5'- -gGCUCCAggagcaccgacCACgGCGuggacucGGCCCAUUCGGc -3' miRNA: 3'- aaCGAGGU-----------GUGaCGC-------CCGGGUAAGCC- -5' |
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| 19028 | 5' | -57.9 | NC_004684.1 | + | 37293 | 1.07 | 0.000883 |
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Target: 5'- gUUGCUCCACACUGCGGGCCCAUUCGGc -3' miRNA: 3'- -AACGAGGUGUGACGCCCGGGUAAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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