Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19033 | 3' | -48.9 | NC_004684.1 | + | 40137 | 0.66 | 0.980231 |
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Target: 5'- cGGCACCGa-----CCUCGGCGaUCCUGu -3' miRNA: 3'- -CUGUGGUacuaaaGGAGUUGC-AGGAC- -5' |
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| 19033 | 3' | -48.9 | NC_004684.1 | + | 65186 | 0.66 | 0.977715 |
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Target: 5'- --gACCAUGuugUCCUCGGCGUagUGg -3' miRNA: 3'- cugUGGUACuaaAGGAGUUGCAggAC- -5' |
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| 19033 | 3' | -48.9 | NC_004684.1 | + | 58687 | 0.66 | 0.971988 |
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Target: 5'- -cCGCCAUGGagUCCUCGuCGUCgUa -3' miRNA: 3'- cuGUGGUACUaaAGGAGUuGCAGgAc -5' |
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| 19033 | 3' | -48.9 | NC_004684.1 | + | 8845 | 0.67 | 0.968757 |
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Target: 5'- cGACugGCCAUGGg--CCUgGACGUgucgCCUGa -3' miRNA: 3'- -CUG--UGGUACUaaaGGAgUUGCA----GGAC- -5' |
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| 19033 | 3' | -48.9 | NC_004684.1 | + | 30879 | 0.67 | 0.968757 |
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Target: 5'- aACGCCGUGcccaggCUCAGCGUCCc- -3' miRNA: 3'- cUGUGGUACuaaag-GAGUUGCAGGac -5' |
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| 19033 | 3' | -48.9 | NC_004684.1 | + | 14007 | 0.67 | 0.957494 |
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Target: 5'- gGACACgGUGcaGUggCCcaacucggUCAACGUCCUGc -3' miRNA: 3'- -CUGUGgUAC--UAaaGG--------AGUUGCAGGAC- -5' |
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| 19033 | 3' | -48.9 | NC_004684.1 | + | 58055 | 0.68 | 0.927344 |
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Target: 5'- cGCACCG-GAggccUCCUCGucccCGUCCUGu -3' miRNA: 3'- cUGUGGUaCUaa--AGGAGUu---GCAGGAC- -5' |
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| 19033 | 3' | -48.9 | NC_004684.1 | + | 43639 | 0.68 | 0.927344 |
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Target: 5'- aGCGCgGUGAgUUCgUCGgaccaaACGUCCUGg -3' miRNA: 3'- cUGUGgUACUaAAGgAGU------UGCAGGAC- -5' |
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| 19033 | 3' | -48.9 | NC_004684.1 | + | 30283 | 0.69 | 0.894123 |
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Target: 5'- cACGCCAgccc-UCCUCAACGUUCUc -3' miRNA: 3'- cUGUGGUacuaaAGGAGUUGCAGGAc -5' |
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| 19033 | 3' | -48.9 | NC_004684.1 | + | 36059 | 1.11 | 0.004403 |
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Target: 5'- cGACACCAUGAUUUCCUCAACGUCCUGg -3' miRNA: 3'- -CUGUGGUACUAAAGGAGUUGCAGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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