Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 19044 | 3' | -55.2 | NC_004684.1 | + | 57820 | 0.71 | 0.52649 |
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Target: 5'- cGGAUCAGGUCGGC-CaCGUguucGCGGc-- -3' miRNA: 3'- -CCUAGUCCAGCUGcG-GCAa---CGCCacu -5' |
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| 19044 | 3' | -55.2 | NC_004684.1 | + | 58566 | 0.69 | 0.63242 |
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Target: 5'- -aGUCGGGcgaUGGCGUCGUUGCGGg-- -3' miRNA: 3'- ccUAGUCCa--GCUGCGGCAACGCCacu -5' |
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| 19044 | 3' | -55.2 | NC_004684.1 | + | 62333 | 0.7 | 0.557784 |
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Target: 5'- uGGAUCAGGaUCGugggaGCGCCGggUGCcagacgcguGGUGGc -3' miRNA: 3'- -CCUAGUCC-AGC-----UGCGGCa-ACG---------CCACU- -5' |
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| 19044 | 3' | -55.2 | NC_004684.1 | + | 64966 | 0.69 | 0.643151 |
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Target: 5'- cGGuUCAGGUCGAUGaCCGcgacGCGGg-- -3' miRNA: 3'- -CCuAGUCCAGCUGC-GGCaa--CGCCacu -5' |
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| 19044 | 3' | -55.2 | NC_004684.1 | + | 65868 | 0.67 | 0.717448 |
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Target: 5'- cGGGUCGcGUCGuCGCCGUUgagcGCGGc-- -3' miRNA: 3'- -CCUAGUcCAGCuGCGGCAA----CGCCacu -5' |
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| 19044 | 3' | -55.2 | NC_004684.1 | + | 65876 | 0.74 | 0.365729 |
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Target: 5'- cGGAg-GGGUUGACGCCGUaGUGGUc- -3' miRNA: 3'- -CCUagUCCAGCUGCGGCAaCGCCAcu -5' |
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| 19044 | 3' | -55.2 | NC_004684.1 | + | 66723 | 0.69 | 0.621689 |
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Target: 5'- aGGAUCAGG-CGcACGCUGUcgUGCGcGUcGGu -3' miRNA: 3'- -CCUAGUCCaGC-UGCGGCA--ACGC-CA-CU- -5' |
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| 19044 | 3' | -55.2 | NC_004684.1 | + | 67194 | 0.67 | 0.738109 |
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Target: 5'- cGGGUCAcGGUCu-CGCCGUUcggcaggucaaCGGUGAa -3' miRNA: 3'- -CCUAGU-CCAGcuGCGGCAAc----------GCCACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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