Results 21 - 28 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19044 | 3' | -55.2 | NC_004684.1 | + | 64966 | 0.69 | 0.643151 |
Target: 5'- cGGuUCAGGUCGAUGaCCGcgacGCGGg-- -3' miRNA: 3'- -CCuAGUCCAGCUGC-GGCaa--CGCCacu -5' |
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19044 | 3' | -55.2 | NC_004684.1 | + | 48043 | 0.69 | 0.653871 |
Target: 5'- cGAUCAGG-CGGCGCa--UGCGGaUGGc -3' miRNA: 3'- cCUAGUCCaGCUGCGgcaACGCC-ACU- -5' |
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19044 | 3' | -55.2 | NC_004684.1 | + | 20985 | 0.68 | 0.706991 |
Target: 5'- cGGcgCAGGUCGGC-CCGauccUGCGGc-- -3' miRNA: 3'- -CCuaGUCCAGCUGcGGCa---ACGCCacu -5' |
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19044 | 3' | -55.2 | NC_004684.1 | + | 65868 | 0.67 | 0.717448 |
Target: 5'- cGGGUCGcGUCGuCGCCGUUgagcGCGGc-- -3' miRNA: 3'- -CCUAGUcCAGCuGCGGCAA----CGCCacu -5' |
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19044 | 3' | -55.2 | NC_004684.1 | + | 67194 | 0.67 | 0.738109 |
Target: 5'- cGGGUCAcGGUCu-CGCCGUUcggcaggucaaCGGUGAa -3' miRNA: 3'- -CCUAGU-CCAGcuGCGGCAAc----------GCCACU- -5' |
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19044 | 3' | -55.2 | NC_004684.1 | + | 42166 | 0.67 | 0.748293 |
Target: 5'- ----uGGGUCGagaGCGCCGcacugcGCGGUGAg -3' miRNA: 3'- ccuagUCCAGC---UGCGGCaa----CGCCACU- -5' |
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19044 | 3' | -55.2 | NC_004684.1 | + | 45763 | 0.66 | 0.787801 |
Target: 5'- aGGuUCuuGGUC-ACGCCGgUGCGGUa- -3' miRNA: 3'- -CCuAGu-CCAGcUGCGGCaACGCCAcu -5' |
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19044 | 3' | -55.2 | NC_004684.1 | + | 15067 | 0.77 | 0.248341 |
Target: 5'- cGGAUgAGGUCGACGgccuuaUCGgcGCGGUGGc -3' miRNA: 3'- -CCUAgUCCAGCUGC------GGCaaCGCCACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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