Results 21 - 29 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19047 | 5' | -56.9 | NC_004684.1 | + | 39608 | 0.67 | 0.660124 |
Target: 5'- aCCgCCGcGCACCGUcgGGCGGGguguacagcccGGGCGa -3' miRNA: 3'- aGG-GGC-UGUGGUAa-CUGCUCa----------CCCGC- -5' |
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19047 | 5' | -56.9 | NC_004684.1 | + | 46916 | 0.69 | 0.533317 |
Target: 5'- aUCCCCcagGACcgGCCAacGGCGGuGUGGGUGa -3' miRNA: 3'- -AGGGG---CUG--UGGUaaCUGCU-CACCCGC- -5' |
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19047 | 5' | -56.9 | NC_004684.1 | + | 51030 | 0.67 | 0.634557 |
Target: 5'- gUCCaCCGGCaggaugcggccaccGCCggUGACGAugcgcccGUGGGUGa -3' miRNA: 3'- -AGG-GGCUG--------------UGGuaACUGCU-------CACCCGC- -5' |
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19047 | 5' | -56.9 | NC_004684.1 | + | 55131 | 0.67 | 0.660124 |
Target: 5'- cCCCCGGCgaaaugGCCGguugUGGCG-GUGGaGCc -3' miRNA: 3'- aGGGGCUG------UGGUa---ACUGCuCACC-CGc -5' |
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19047 | 5' | -56.9 | NC_004684.1 | + | 55957 | 0.7 | 0.482773 |
Target: 5'- -gCCCGGucaGCUccggGUUGGCGaAGUGGGCGg -3' miRNA: 3'- agGGGCUg--UGG----UAACUGC-UCACCCGC- -5' |
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19047 | 5' | -56.9 | NC_004684.1 | + | 56885 | 0.69 | 0.492706 |
Target: 5'- gCCCCG-CACC-UUGGCGAG-GaGGCc -3' miRNA: 3'- aGGGGCuGUGGuAACUGCUCaC-CCGc -5' |
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19047 | 5' | -56.9 | NC_004684.1 | + | 61680 | 0.72 | 0.347403 |
Target: 5'- -gCCUGcACGCCAUUGGCGucggugccGUGGGCa -3' miRNA: 3'- agGGGC-UGUGGUAACUGCu-------CACCCGc -5' |
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19047 | 5' | -56.9 | NC_004684.1 | + | 64691 | 0.67 | 0.64735 |
Target: 5'- cCCCCGGCACCGgccaggccgggGAUGcGGUGcccGGCGc -3' miRNA: 3'- aGGGGCUGUGGUaa---------CUGC-UCAC---CCGC- -5' |
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19047 | 5' | -56.9 | NC_004684.1 | + | 66338 | 0.67 | 0.638822 |
Target: 5'- gUCCCggUGACGCCgg-GGCGGGcggccaGGGCGa -3' miRNA: 3'- -AGGG--GCUGUGGuaaCUGCUCa-----CCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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