Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19047 | 5' | -56.9 | NC_004684.1 | + | 5388 | 0.69 | 0.523043 |
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Target: 5'- gUCCCCGuCGCgAUUGugcgcgGCGGGUgcGGGUGg -3' miRNA: 3'- -AGGGGCuGUGgUAAC------UGCUCA--CCCGC- -5' |
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| 19047 | 5' | -56.9 | NC_004684.1 | + | 39153 | 0.69 | 0.502732 |
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Target: 5'- gUCCaCCG-CGCUGUgcucCGGGUGGGCGg -3' miRNA: 3'- -AGG-GGCuGUGGUAacu-GCUCACCCGC- -5' |
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| 19047 | 5' | -56.9 | NC_004684.1 | + | 56885 | 0.69 | 0.492706 |
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Target: 5'- gCCCCG-CACC-UUGGCGAG-GaGGCc -3' miRNA: 3'- aGGGGCuGUGGuAACUGCUCaC-CCGc -5' |
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| 19047 | 5' | -56.9 | NC_004684.1 | + | 55957 | 0.7 | 0.482773 |
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Target: 5'- -gCCCGGucaGCUccggGUUGGCGaAGUGGGCGg -3' miRNA: 3'- agGGGCUg--UGG----UAACUGC-UCACCCGC- -5' |
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| 19047 | 5' | -56.9 | NC_004684.1 | + | 34627 | 0.7 | 0.463204 |
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Target: 5'- uUCCCCGACGagacccuugcCCGUcaGAUG-GUGGGCa -3' miRNA: 3'- -AGGGGCUGU----------GGUAa-CUGCuCACCCGc -5' |
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| 19047 | 5' | -56.9 | NC_004684.1 | + | 29174 | 0.7 | 0.463204 |
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Target: 5'- gUUCCCGAaccuucaGCCGUgGACGcAGUGGGUc -3' miRNA: 3'- -AGGGGCUg------UGGUAaCUGC-UCACCCGc -5' |
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| 19047 | 5' | -56.9 | NC_004684.1 | + | 61680 | 0.72 | 0.347403 |
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Target: 5'- -gCCUGcACGCCAUUGGCGucggugccGUGGGCa -3' miRNA: 3'- agGGGC-UGUGGUAACUGCu-------CACCCGc -5' |
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| 19047 | 5' | -56.9 | NC_004684.1 | + | 30767 | 0.79 | 0.132243 |
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Target: 5'- aCCCCGACGagGUgGACGGGUGGGUGc -3' miRNA: 3'- aGGGGCUGUggUAaCUGCUCACCCGC- -5' |
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| 19047 | 5' | -56.9 | NC_004684.1 | + | 28037 | 1.1 | 0.000863 |
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Target: 5'- uUCCCCGACACCAUUGACGAGUGGGCGg -3' miRNA: 3'- -AGGGGCUGUGGUAACUGCUCACCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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