Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 19050 | 3' | -63.9 | NC_004684.1 | + | 52545 | 0.68 | 0.256114 |
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Target: 5'- gAGGUGUCCGGcaccGGUucGUAGGuGAUGGUGa -3' miRNA: 3'- -UCCACGGGCC----UCG--CGUCCcCUACCGC- -5' |
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| 19050 | 3' | -63.9 | NC_004684.1 | + | 6447 | 0.67 | 0.275159 |
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Target: 5'- ---cGCuuGGccgaGGCGCAGGGGAUgaugaccagcugGGCGg -3' miRNA: 3'- uccaCGggCC----UCGCGUCCCCUA------------CCGC- -5' |
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| 19050 | 3' | -63.9 | NC_004684.1 | + | 36999 | 0.67 | 0.288475 |
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Target: 5'- aAGGUGUacauCCGGcGCGCGGGGuccGAUGaGCc -3' miRNA: 3'- -UCCACG----GGCCuCGCGUCCC---CUAC-CGc -5' |
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| 19050 | 3' | -63.9 | NC_004684.1 | + | 16753 | 0.67 | 0.302292 |
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Target: 5'- cGGcugGCCUGGAacuCGC-GGGGAUGaGCGg -3' miRNA: 3'- uCCa--CGGGCCUc--GCGuCCCCUAC-CGC- -5' |
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| 19050 | 3' | -63.9 | NC_004684.1 | + | 19775 | 0.67 | 0.30939 |
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Target: 5'- cGGUGCCCgccgGGGGCGaCAa--GAUGGCGg -3' miRNA: 3'- uCCACGGG----CCUCGC-GUcccCUACCGC- -5' |
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| 19050 | 3' | -63.9 | NC_004684.1 | + | 47100 | 0.75 | 0.082654 |
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Target: 5'- cGGUGCCgGGA-CGCGGGGGuccGGCa -3' miRNA: 3'- uCCACGGgCCUcGCGUCCCCua-CCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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