Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19050 | 3' | -63.9 | NC_004684.1 | + | 40716 | 0.72 | 0.125997 |
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Target: 5'- cGGUGgCCGGgacgaccAGCGCggcgGGGGcGGUGGCGg -3' miRNA: 3'- uCCACgGGCC-------UCGCG----UCCC-CUACCGC- -5' |
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| 19050 | 3' | -63.9 | NC_004684.1 | + | 39959 | 0.73 | 0.110188 |
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Target: 5'- cGGUGCCCGGAGCGCcauccccugcgcuccGGGcaccuggucgguguGGUGGCu -3' miRNA: 3'- uCCACGGGCCUCGCGu--------------CCC--------------CUACCGc -5' |
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| 19050 | 3' | -63.9 | NC_004684.1 | + | 44924 | 0.73 | 0.10903 |
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Target: 5'- cAGGgaaccgGCCUGGAGCGCGGgcGGGAUcgccgccgcgccgacGGCGa -3' miRNA: 3'- -UCCa-----CGGGCCUCGCGUC--CCCUA---------------CCGC- -5' |
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| 19050 | 3' | -63.9 | NC_004684.1 | + | 22196 | 0.75 | 0.082654 |
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Target: 5'- --cUGCCgcUGGAGCGCGGcGGGGUGGUGg -3' miRNA: 3'- uccACGG--GCCUCGCGUC-CCCUACCGC- -5' |
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| 19050 | 3' | -63.9 | NC_004684.1 | + | 47100 | 0.75 | 0.082654 |
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Target: 5'- cGGUGCCgGGA-CGCGGGGGuccGGCa -3' miRNA: 3'- uCCACGGgCCUcGCGUCCCCua-CCGc -5' |
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| 19050 | 3' | -63.9 | NC_004684.1 | + | 27417 | 1.07 | 0.000277 |
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Target: 5'- cAGGUGCCCGGAGCGCAGGGGAUGGCGc -3' miRNA: 3'- -UCCACGGGCCUCGCGUCCCCUACCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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