Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 19050 | 5' | -55 | NC_004684.1 | + | 8838 | 0.66 | 0.749201 |
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Target: 5'- cAUCGCCcgacUGGCCAUGGGccuGGACGUg- -3' miRNA: 3'- -UGGCGGu---AUUGGUGUCCu--CCUGCAgu -5' |
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| 19050 | 5' | -55 | NC_004684.1 | + | 52240 | 0.67 | 0.72841 |
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Target: 5'- gGCCGUgGU-GCCACAGcGGcGGuACGUCGa -3' miRNA: 3'- -UGGCGgUAuUGGUGUC-CU-CC-UGCAGU- -5' |
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| 19050 | 5' | -55 | NC_004684.1 | + | 45694 | 0.67 | 0.717867 |
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Target: 5'- gGCCgaGCCAUGACCACGGuGucgcacaucGGGAaGUCGg -3' miRNA: 3'- -UGG--CGGUAUUGGUGUC-C---------UCCUgCAGU- -5' |
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| 19050 | 5' | -55 | NC_004684.1 | + | 36450 | 0.67 | 0.696543 |
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Target: 5'- uGCUGgCGUGACCAU-GGuGGACGUgAg -3' miRNA: 3'- -UGGCgGUAUUGGUGuCCuCCUGCAgU- -5' |
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| 19050 | 5' | -55 | NC_004684.1 | + | 62455 | 0.68 | 0.685784 |
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Target: 5'- cCUGCCAgcGCUcCGGGAGGugGcCGu -3' miRNA: 3'- uGGCGGUauUGGuGUCCUCCugCaGU- -5' |
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| 19050 | 5' | -55 | NC_004684.1 | + | 15880 | 0.68 | 0.653246 |
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Target: 5'- gGCCGCCAgcgGCCcuguGCAGccgcAGGGCGUCc -3' miRNA: 3'- -UGGCGGUau-UGG----UGUCc---UCCUGCAGu -5' |
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| 19050 | 5' | -55 | NC_004684.1 | + | 19332 | 0.77 | 0.225206 |
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Target: 5'- cGCCGCCGUGGCCgaGCGGGAGG-CGc-- -3' miRNA: 3'- -UGGCGGUAUUGG--UGUCCUCCuGCagu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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