Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19054 | 3' | -52.6 | NC_004684.1 | + | 66399 | 0.67 | 0.847813 |
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Target: 5'- uGGUgUCCUCCUcGGgcUggCGGGuCCGu -3' miRNA: 3'- gCUAgAGGAGGA-CCauAaaGUCC-GGC- -5' |
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| 19054 | 3' | -52.6 | NC_004684.1 | + | 63709 | 0.67 | 0.844357 |
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Target: 5'- gCGAUCUCCUgCCagcgugccaacagGGUGUgcgcaUCGGuGCCGa -3' miRNA: 3'- -GCUAGAGGA-GGa------------CCAUAa----AGUC-CGGC- -5' |
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| 19054 | 3' | -52.6 | NC_004684.1 | + | 64834 | 0.68 | 0.802189 |
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Target: 5'- uGGUgUCCUCCuUGGUAgg-CGGGUgGu -3' miRNA: 3'- gCUAgAGGAGG-ACCAUaaaGUCCGgC- -5' |
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| 19054 | 3' | -52.6 | NC_004684.1 | + | 40646 | 0.68 | 0.782616 |
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Target: 5'- uCGGUCaUCUCCaucagGGUGUcgCGGGCCu -3' miRNA: 3'- -GCUAGaGGAGGa----CCAUAaaGUCCGGc -5' |
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| 19054 | 3' | -52.6 | NC_004684.1 | + | 59275 | 0.69 | 0.772588 |
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Target: 5'- gCGGUCgUCCUCgUcaaugcugccGGUGgg-CAGGCCGg -3' miRNA: 3'- -GCUAG-AGGAGgA----------CCAUaaaGUCCGGC- -5' |
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| 19054 | 3' | -52.6 | NC_004684.1 | + | 25763 | 1.09 | 0.002638 |
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Target: 5'- gCGAUCUCCUCCUGGUAUUUCAGGCCGc -3' miRNA: 3'- -GCUAGAGGAGGACCAUAAAGUCCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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