miRNA: 3'- -GCGGAGUUGCauuaCa---GCCACCG--CGc -5'
miRNA: 3'- -GCGGa--GU---UGCAUUACAGc-CACCGCGc -5'
miRNA: 3'- gCGGAGUUGCauUaca-GCCACC-GCGC- -5'
miRNA: 3'- -GCGGAGUUGCAUUACAGCCACCGCGC- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19068 | 3' | -54.1 | NC_004684.1 | + | 10686 | 0.74 | 0.387106 |
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Target: 5'- aCGCCUCAACGgucgGgacgCGGUGGCucGCa -3' miRNA: 3'- -GCGGAGUUGCauuaCa---GCCACCG--CGc -5' |
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| 19068 | 3' | -54.1 | NC_004684.1 | + | 13644 | 0.76 | 0.297926 |
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Target: 5'- cCGCCgcgCAccuGCGUGGUGUCucGUGGCGCa -3' miRNA: 3'- -GCGGa--GU---UGCAUUACAGc-CACCGCGc -5' |
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| 19068 | 3' | -54.1 | NC_004684.1 | + | 33471 | 0.77 | 0.269691 |
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Target: 5'- uGCCUCGGCGgcGgcugCGGUGGaCGCGg -3' miRNA: 3'- gCGGAGUUGCauUaca-GCCACC-GCGC- -5' |
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| 19068 | 3' | -54.1 | NC_004684.1 | + | 20668 | 1.09 | 0.002007 |
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Target: 5'- cCGCCUCAACGUAAUGUCGGUGGCGCGc -3' miRNA: 3'- -GCGGAGUUGCAUUACAGCCACCGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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